Consensus sequence engineering of potato patatin: Enhancing recombinant production and functional properties
Jinsong Zhao1, Qingtong Wu1, Xinyu Guan1
1National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A & F University, Hangzhou, Zhejiang 311300, China.
Bioresource Technology
|June 9, 2026
Summary
Engineered potato patatin (ConPatatin) achieves fivefold higher yields and enhanced stability and activity. This breakthrough offers a sustainable method for industrial patatin production and protein design.
Area of Science:
- Biochemistry
- Protein Engineering
- Food Science
Background:
- Patatin, a potato protein, has valuable properties for food and biomaterials.
- Traditional patatin extraction is inefficient and slow, limiting commercial use.
Purpose of the Study:
- To engineer a novel patatin variant (ConPatatin) for efficient and stable production.
- To enhance the functional properties of patatin for broader applications.
Main Methods:
- Consensus sequence engineering was used to design ConPatatin.
- Recombinant protein expression and characterization were performed.
- Mechanistic studies identified key residues for soluble expression.
Main Results:
- ConPatatin achieved yields up to 200 mg/L, five times higher than other recombinant patatins.
- Recombinant ConPatatin demonstrated high solubility, structural integrity, and enzymatic stability.
- Enhanced antioxidant and esterase activities were observed compared to native and other recombinant forms.
Conclusions:
- Consensus sequence engineering is effective for improving protein production and function.
- ConPatatin offers a sustainable and efficient source for industrial applications.
- The findings provide a reference for rational protein design and optimization.
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