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Correlation between serum and plasma lipoprotein(a) measurements: ARIC study
Yakubu Bene-Alhasan1, Caroline Sun1, Arsalan Hamid1
1Section of Cardiovascular Research, Department of Medicine, Baylor College of Medicine, Houston, TX, USA (Bene-Alhasan, Sun, Hamid, Nambi, Ballantyne, and Hoogeveen).
Journal of Clinical Lipidology
|June 9, 2026
Summary
Serum and plasma lipoprotein(a) [Lp(a)] measurements show excellent agreement. This finding supports using either sample type for Lp(a) testing and combining data from both sources in research.
Area of Science:
- Cardiovascular Research
- Biomarker Analysis
- Clinical Chemistry
Background:
- Lipoprotein(a) [Lp(a)] is crucial for atherosclerotic cardiovascular disease (ASCVD) risk assessment.
- Emerging therapies target Lp(a), necessitating reliable measurement methods.
- Previous studies relied on plasma, but serum is often more accessible.
Purpose of the Study:
- To evaluate the concordance between serum and plasma Lp(a) levels.
- To validate the use of serum samples for Lp(a) quantification.
Main Methods:
- Lp(a) was measured in both EDTA plasma and serum from 100 Atherosclerosis Risk in Communities participants.
- A validated, isoform-insensitive assay was employed for all measurements.
- Statistical analyses included Spearman correlation, Deming regression, and Bland-Altman analysis.
Main Results:
- An excellent correlation (Spearman's r = 0.99) was observed between serum and plasma Lp(a).
- Deming regression showed a slope of 1.05 (95% CI 1.03, 1.08), indicating minimal systematic difference.
- Bland-Altman analysis revealed no statistically significant mean difference between the two sample types.
Conclusions:
- Serum and plasma Lp(a) measurements demonstrate high agreement.
- Either sample type can be reliably used for Lp(a) quantification.
- Data from serum and plasma samples can be pooled for comprehensive Lp(a) analysis.
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