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Updated: Jun 11, 2026

BioMEMS: Forging New Collaborations Between Biologists and Engineers

BioMEMS: Forging New Collaborations Between Biologists and Engineers

Published on: November 1, 2007

A microfluidic platform for whole-membrane integrity profiling in live neuronal cells.

Till Ryser¹, Ata Krichene¹, Nicolò Marchi¹

¹Laboratory of Life Science Electronics, École polytechnique fédérale de Lausanne (EPFL), Lausanne, Switzerland.

Microsystems & Nanoengineering

|June 9, 2026

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This study introduces a novel microfluidic platform for real-time, label-free assessment of cellular membrane integrity. The system quantifies how different forms of alpha-Synuclein impact neuronal membranes at the single-cell level.

Area of Science:

Cell Biology
Neuroscience
Biophysics

Background:

Cellular membrane integrity is crucial for health and disease, particularly in neurodegenerative disorders like Parkinson's disease.
Current methods for assessing membrane integrity are limited in dynamic, whole-cell, and single-cell measurements.
There is a need for advanced techniques to monitor membrane integrity over time across the entire cell surface.

Purpose of the Study:

To develop and apply a microfluidic platform for real-time, label-free assessment of membrane integrity.
To investigate the effects of different aggregated forms of alpha-Synuclein (aSyn) on neuronal membranes.
To enable dynamic, single-cell, whole-membrane analysis of membrane disruption.

Main Methods:

A microfluidic platform integrating electrokinetic microdevices with 3D microelectrodes and imaging was developed.

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Last Updated: Jun 11, 2026

BioMEMS: Forging New Collaborations Between Biologists and Engineers

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Published on: November 1, 2007

Microfluidic Chip for Axonal Injury Models Construction and Enabling Multi-Omics Analysis

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Published on: October 14, 2025

The system allows continuous analysis of live neuronal cells in flow.

Electrorotation responses were measured to quantify changes in plasma membrane capacitance.

Main Results:

The platform successfully assessed membrane integrity in real-time and label-free.
Different aggregated forms of alpha-Synuclein (monomeric, oligomeric, fibrillar) exhibited distinct membrane-disruptive effects.
The study achieved single-cell resolution and whole-membrane sensitivity in quantifying these effects.

Conclusions:

The developed microfluidic platform offers a powerful tool for studying membrane integrity in cellular models.
This method provides a time-resolved comparison of the membrane-disruptive potential of various alpha-Synuclein conformations.
The findings contribute to understanding the role of alpha-Synuclein in Parkinson's disease pathogenesis at the membrane level.