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Related Experiment Video

Updated: Jun 11, 2026

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

High-Throughput In-Depth Single-Cell Lipidomics by Ozone-Induced Dissociation and Mass Cytometry.

Yunyun Yang1, Haishen Zeng2, Yuehua Wu3

  • 1Guangdong Provincial Key Laboratory of Chemical Measurement and Emergency Test Technology, Guangdong Provincial Engineering Research Center for Ambient Mass Spectrometry, Institute of Analysis, Guangdong Academy of Sciences (China National Analytical Center, Guangzhou), Guangzhou 510070, China.

Analytical Chemistry
|June 10, 2026
PubMed
Summary

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This summary is machine-generated.

Researchers developed a high-throughput single-cell lipidomics platform using ozone-induced dissociation (OzID) and mass cytometry. This method precisely analyzes lipids and their structures, enabling cell type discrimination and biomarker discovery.

Area of Science:

  • Biochemistry
  • Cell Biology
  • Analytical Chemistry

Background:

  • Lipids are crucial for cellular structure and function.
  • Single-cell lipidomics offers deep insights into cellular processes.
  • Existing methods lack high throughput for comprehensive lipid analysis.

Purpose of the Study:

  • To develop a high-throughput single-cell lipidomics platform.
  • To integrate ozone-induced dissociation (OzID) with mass cytometry.
  • To enable in-depth analysis of intact lipids and their structural information.

Main Methods:

  • Coupling ozone-induced dissociation (OzID) with mass cytometry.
  • Optimizing OzID conditions to cleave unsaturated lipid C═C bonds.
  • Performing high-throughput single-cell mass cytometry analysis.

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Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
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Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

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Last Updated: Jun 11, 2026

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage
08:07

Sample Preparation for Single Cell Mass Spectrometry Metabolomics Studies: Combined Cell Washing, Quenching, Drying, and Storage

Published on: September 16, 2025

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures
09:38

Single-throughput Complementary High-resolution Analytical Techniques for Characterizing Complex Natural Organic Matter Mixtures

Published on: January 7, 2019

Main Results:

  • Achieved a throughput of 166 cells/min.
  • Generated diagnostic products from lipid C═C bond cleavage.
  • Successfully discriminated between cell types with similar intact lipid profiles (e.g., lung epithelial vs. cancer cells).

Conclusions:

  • The developed platform provides in-depth single-cell lipidomics.
  • It enables the analysis of both intact lipids and C═C bond information.
  • The platform shows significant potential for cell type discrimination and novel lipid biomarker discovery.