Comparison of Laparotomy and Percutaneous Intraperitoneal Lavage on Survival in a Cecal Slurry Peritonitis Model in...

We aimed to compare survival rates in a 4-day-old mouse model of cecal slurry (CS)-induced bacterial peritonitis managed by laparotomy (LAP) or percutaneous peritoneal lavage (PPL). Four-day-old pups received intraperitoneal injections of CS to induce severe (3.0 g/gBW) or mild (1.0 g/gBW) peritonitis. Two hours later, pups underwent either LAP or PPL. Seven-day survival rates, perioperative body temperature (BT) changes, and postoperative ascites and plasma cytokine levels were assessed. In the severe peritonitis group, the 7-day survival rate tended to be higher in pups who underwent PPL than in those who underwent LAP (36.4% vs. 9.1%, p = 0.069). In the mild peritonitis group, survival rates were similar between treatments (83.3% vs. 72.7%, p = 0.53). The median perioperative BT change was significantly smaller in pups who underwent PPL for both the severe (-1.2℃ vs. -2.6℃, p < 0.01) and mild (-0.2℃ vs. -2.9℃, p < 0.01) peritonitis groups. Postoperative tumor necrosis factor levels were significantly lower in the PPL group compared with the LAP group under mild peritonitis (202 pg/ml vs. 443 pg/ml, p = 0.034). We established a novel 4-day-old PPL mouse model and identified PPL as a potential minimally invasive alternative that might help prevent perioperative hypothermia in the fatal peritonitis.