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Related Experiment Video

Updated: Jun 11, 2026

Fluorescence-quenching of a Liposomal-encapsulated Near-infrared Fluorophore as a Tool for In Vivo Optical Imaging
10:55

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Published on: January 5, 2015

Making Fluorescent Nylon, Polypropylene, and Polystyrene Microplastics for In Vivo and In Vitro Imaging.

Charles E Bardawil1, Jarrett Dobbins1, Shannon Lankford1

  • 1Department of Cardiothoracic Surgery, Wake Forest University School of Medicine, Winston-Salem, NC 27157, USA.

Microplastics (Basel, Switzerland)
|June 10, 2026
PubMed
Summary
This summary is machine-generated.

Researchers developed two methods to fluorescently label microplastics (MPs) for tracking biological impacts. These techniques allow visualization and quantification of MPs in vivo, aiding health effect studies.

Keywords:
fluorescencein vivo imagingmicroplasticsmicroscopynylonpolypropylenepolystyrene

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Area of Science:

  • Environmental Science
  • Toxicology
  • Biomedical Imaging

Background:

  • Microplastics (MPs) are prevalent environmental pollutants with potential adverse human health effects.
  • Tracking MPs in biological systems is crucial for understanding their impact, but ambient MPs lack intrinsic fluorescence.
  • Existing methods for MP visualization in vivo are limited.

Purpose of the Study:

  • To develop and validate novel fluorescent labeling strategies for microplastics.
  • To enable visualization, tracking, and quantification of microplastics in biological systems.
  • To facilitate research into the health implications of microplastic exposure.

Main Methods:

  • Staining nylon and polypropylene MPs with Rhodamine 6G for fluorescent microscopy.
  • Conjugating aminated polystyrene microspheres with IRDye-800CW for near-infrared imaging.
  • In vivo tracking of labeled MPs in murine models using fluorescent and near-infrared imaging systems.

Main Results:

  • Rhodamine 6G-labeled MPs showed stable fluorescence in murine lung tissue for up to one week.
  • IRDye-800CW-labeled MPs enabled high-resolution imaging with minimal autofluorescence.
  • In vivo studies detected organ-specific accumulation of IRDye-labeled MPs in the liver and spleen up to 72 hours post-injection.

Conclusions:

  • Developed practical fluorescent labeling strategies for microplastics.
  • Enabled visualization and tracking of MPs in biological systems.
  • Advanced tools for studying microplastic biodistribution and health effects.