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Updated: Jun 11, 2026

Sequence-specific Labeling of Nucleic Acids and Proteins with Methyltransferases and Cofactor Analogues
Published on: November 22, 2014
Site-specific labelling of native peptides and proteins: chemical and enzymatic strategies
Antonio Angelastro1, Jonathan Bargh2, Subhajit Guria3
1Biologics Engineering, Oncology R&D, Cambridge Biomedical Campus, AstraZeneca, 1 Francis Crick Ave, Trumpington, Cambridge, CB2 0AA, UK.
Abstract:
Site-specific modifications of native-sequence proteins are technologies that underpin progresses in chemical biology, diagnostics and next-generation biotherapeutics. However, the pursuit of site-specificity has often come at the expense of scalability and usability, ultimately limiting translational potential of a modification tool. This review critically compares current key strategies, including terminal, disulfide-rebridging, small-molecule, glycan and enzyme-based modifications. Finally, we present a decision tree for method selection and highlight opportunities for innovation in next-generation native-sequence protein modification technologies.
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