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Related Experiment Video

Updated: Jun 11, 2026

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

Published on: July 30, 2014

Versatile vector tools for efficient protein screening across multiple expression systems.

Zhimin Zhu¹, Yaqing Liu², Lei Qin³

¹Shanghai Institute of Applied Physics, Chinese Academy of Sciences, Shanghai, China.

|June 10, 2026

Summary

This summary is machine-generated.

Researchers developed versatile vector tools for rapid heterologous protein expression screening across E. coli, insect, and mammalian cells. This streamlines gene construction and protein purification, significantly improving research efficiency.

Keywords:

eukaryotic expression systems high‐throughput screening multi‐host protein expression vector toolkit

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Last Updated: Jun 11, 2026

High Throughput Quantitative Expression Screening and Purification Applied to Recombinant Disulfide-rich Venom Proteins Produced in E. coli

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Published on: July 30, 2014

Pulldown Assay Coupled with Co-Expression in Bacteria Cells as a Time-Efficient Tool for Testing Challenging Protein-Protein Interactions

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Published on: December 23, 2022

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Recombinant Protein Expression for Structural Biology in HEK 293F Suspension Cells: A Novel and Accessible Approach

Published on: October 16, 2014

Area of Science:

Molecular Biology
Biotechnology
Protein Science

Background:

Heterologous protein expression is crucial for biological research.
Screening across diverse expression systems (E. coli, insect, mammalian) is typically labor-intensive and time-consuming.

Purpose of the Study:

To develop versatile vector tools that standardize and accelerate the process of screening protein expression across multiple systems.
To enhance the efficiency of parallel protein screening and facilitate protein purification.

Main Methods:

Development of standardized vector tools compatible with E. coli, insect, and mammalian cells.
Incorporation of His, MBP, and GST tags for versatile protein purification.
Utilized a standardized interface enabling rapid vector switching via homologous recombination with a single primer pair for PCR.

Main Results:

Demonstrated successful protein expression and purification in E. coli, insect, and mammalian cell systems.
Showcased the ability of the vector tools to significantly accelerate gene construction.
Validated the increased efficiency of parallel protein screening and streamlined purification processes.

Conclusions:

The developed vector tools offer a versatile and effective solution for rapid protein screening across multiple expression systems.
These tools significantly enhance research efficiency by simplifying vector switching and accelerating gene construction.
The standardized approach facilitates high-throughput protein expression and purification, benefiting biological research.