Versatile vector tools for efficient protein screening across multiple expression systems

Heterologous protein expression is a cornerstone of biological research, but screening across different expression systems is often labor-intensive. We developed versatile vector tools for E. coli, insect, and mammalian cells, offering His, MBP, and GST tags. These tools feature a standardized interface that enables rapid vector switching via homologous recombination, requiring only a single pair of primers for PCR amplification. This significantly accelerates gene construction, thereby greatly increasing the efficiency of parallel protein screening. We demonstrate successful protein expression and purification in various systems using these tools, showcasing their ability to streamline protein screening and improve efficiency. Our results highlight the versatility and effectiveness of these vector tools in facilitating rapid protein screening across multiple expression systems.