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Cryo-electron Microscopy

Cryo-electron Microscopy

Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...

Overview of Electron Microscopy

Overview of Electron Microscopy

The wavelengths of visible light ultimately limit the maximum theoretical resolution of images created by light microscopes. Most light microscopes can only magnify 1000X, and a few can magnify up to 1500X. Electrons, like electromagnetic radiation, can behave like waves, but with wavelengths of 0.005 nm, they produce significantly greater resolution up to 0.05 nm as compared to 500 nm for visible light. An electron microscope (EM) can create a sharp image that is magnified up to 2,000,000X.

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Related Experiment Video

Updated: Jun 12, 2026

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

Atomic resolution cryo-EM at 200 keV.

Radostin Danev¹, Haruaki Yanagisawa¹, Keitaro Yamashita²

¹Graduate School of Medicine, The University of Tokyo, 7-3-1 Hongo, Bunkyo-ku, Tokyo, 113-0033, Japan.

|June 10, 2026

Summary

This summary is machine-generated.

Researchers achieved 1.24 Å atomic resolution using an upgraded 200 kV cryo-electron microscope. This cost-effective platform rivals 300 kV systems, enabling high-resolution single-particle cryo-EM.

Keywords:

100 kV electron cryo-microscopes 200 kV electron cryo-microscopes apoferritin atomic resolution cryo-EM

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Related Experiment Videos

Last Updated: Jun 12, 2026

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Routine Collection of High-Resolution cryo-EM Datasets Using 200 KV Transmission Electron Microscope

Published on: March 16, 2022

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

A Robust Single-Particle Cryo-Electron Microscopy (cryo-EM) Processing Workflow with cryoSPARC, RELION, and Scipion

Published on: January 31, 2022

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Single-Particle Cryo-EM Data Collection with Stage Tilt using Leginon

Published on: July 1, 2022

Area of Science:

Structural Biology
Microscopy

Background:

Atomic resolution in cryo-electron microscopy (cryo-EM) was first achieved six years ago.
Previous high-resolution cryo-EM utilized 300 kV electron microscopes with specialized hardware.

Purpose of the Study:

To report the achievement of 1.24 Å atomic resolution on an upgraded 200 kV cryo-electron microscope.
To demonstrate a cost-effective single-particle cryo-EM platform with performance comparable to more expensive systems.

Main Methods:

Utilized an upgraded 200 kV electron microscope with a cold field emission gun, high-resolution objective lens polepiece, and energy filter.
Operated the microscope at 100 kV and 200 kV.
Employed a high-speed hybrid-pixel detector.

Main Results:

Achieved 1.24 Å atomic resolution on the upgraded 200 kV microscope.
Demonstrated sub-2 Å resolution at 100 kV using the high-speed detector.
The upgraded 200 kV system offers performance comparable to 300 kV instruments.

Conclusions:

An upgraded 200 kV cryo-EM platform can achieve atomic resolution.
This system provides a cost-effective alternative to higher-voltage microscopes for single-particle analysis.
Further advancements in detector technology enhance resolution capabilities.