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Updated: Jun 12, 2026

Porcine Normothermic Isolated Liver Perfusion

Porcine Normothermic Isolated Liver Perfusion

Published on: June 9, 2023

Oxygenated Perfusion Enhances Hepatocyte Function in Human iPSC-liver Tissue.

Soichiro Yamabe^1,2, Yoshiki Kuse^1,3, Takashi Okumura¹

¹Division of Regenerative Medicine, The Institute of Medical Science, The University of Tokyo, Tokyo, Japan.

Transplantation

|June 10, 2026

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Researchers developed an oxygenation system using decellularized livers and artificial red blood cells to create transplantable human liver tissue from stem cells. This method improves the function and engraftment of engineered liver organoids, offering a promising alternative for organ transplantation.

Area of Science:

Regenerative Medicine
Stem Cell Biology
Bioengineering

Background:

Organ transplantation is limited by donor organ scarcity.
Human induced pluripotent stem cell (hiPSC)-derived organoids offer potential for generating transplantable tissues.
Decellularized liver (DL) scaffolds provide extracellular matrix for cell support and perfusion.

Purpose of the Study:

To create functional and scalable human liver tissue from hiPSC-derived liver organoids (hiPSC-LOs).
To establish an effective oxygenation system for hiPSC-LOs within a DL scaffold.
To improve the viability and function of engineered liver tissue for potential transplantation.

Main Methods:

Refined culture conditions for hiPSC-LOs to enhance engraftment in rat DLs.

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Related Experiment Videos

Last Updated: Jun 12, 2026

Porcine Normothermic Isolated Liver Perfusion

Porcine Normothermic Isolated Liver Perfusion

Published on: June 9, 2023

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion

Functional Human Liver Preservation and Recovery by Means of Subnormothermic Machine Perfusion

Published on: April 27, 2015

An Open-Source Normothermic Perfusion System Designed for Research Scientists

An Open-Source Normothermic Perfusion System Designed for Research Scientists

Published on: July 18, 2025

Developed an oxygenation system using perfusion of oxygen-enriched medium with artificial red blood cells for hiPSC-liver tissue (hiPSC-LT).

Transplanted oxygenated hiPSC-LTs onto immunodeficient mice to assess in vivo performance.

Main Results:

Successfully generated functional hiPSC-LTs by infusing hiPSC-LOs into DLs.
Oxygenation suppressed cell death and promoted hepatic function by mimicking physiological oxygen levels.
Oxygenated hiPSC-LTs showed improved in vivo engraftment, indicated by higher human albumin levels in recipient serum.

Conclusions:

The developed oxygenation system effectively supports the generation of transplantable, functional hiPSC-LT.
This strategy enhances the growth and functionality of hiPSC-organoids.
Represents a significant advancement towards alternatives for conventional organ transplantation.