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Updated: Jun 12, 2026

High-Resolution Three-Dimensional Whole-Organ Tomography of Microbial Infections
Published on: March 1, 2024
Classification of bacterial images obtained optically using some pre-trained models
Lizette Nange Chia1, Thomas Tamo Tatietse2, Gemma Piella3
1Department of Physics, Research Unit of Condensed Matter, Electronics and Signal Processing, Faculty of Sciences, University of Dschang, P.O. Box 67, Cameroon; African Institute of Mathematical Sciences (AIMS), Limbe, P.O. Box 608, Cameroon.
Abstract:
Rapid and accurate detection of water-borne bacteria is critical for safeguarding public health and preventing the spread of infections. Conventional bacterial identification techniques are often time-consuming, resource-intensive, and require specialized personnel, limiting their suitability for automated water-quality monitoring. This study presents an optical detection and classification framework that uses some pre-trained architectures to automatically classify optically acquired images of water-borne bacterial pathogens. The system distinguishes among four classes: Escherichia coli (E. coli), Fecal streptococci (Strept), co-occurrence of both bacteria (Both), and safe water (None). Experimental evaluation demonstrates strong classification performance, with ResNet-50, ResNet-152, and EfficientNet-B7 achieving accuracies of 94.15%, 94.45%, and 95.54%, respectively. Except DenseNet-201, which yields the worst results (accuracy of 77.49%), the corresponding precision, recall, and F1-scores of the other pre-trained models exceed 94%. Furthermore, an analysis of the ROC (Receiver Operating Characteristic) curve reveals that the Area Under the Curve (AUC) exceeds 98% for each bacterial class, thereby demonstrating strong discriminative performance. The results highlight the potential of transfer learning-based convolutional neural networks for accurate, rapid, and cost-effective bacterial detection, emphasizing their promise for scalable and automated water-quality monitoring systems. This approach represents a step toward improved monitoring tools aligned with sustainable water, sanitation, and hygiene (WASH) objectives.
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