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Published on: April 28, 2016
Processing methods modulate the multi-scale digestive behavior of shrimp: protein degradation and peptide release
Jieqian Zhou1, Kyi Kyi Htwe1, Yantao Yin1
1College of Food Science and Technology, Guangdong Ocean University, Guangdong Provincial Key Laboratory of Aquatic Product Processing and Safety, Guangdong Province Engineering Laboratory for Marine Biological Products, Guangdong Provincial Engineering Technology Research Center of Seafood, Guangdong Provincial Engineering Technology Research Center of Prefabricated Seafood Processing and Quality Control, Zhanjiang 524088, China.
None:
Shrimp processing alters protein digestion characteristics. This study systematically investigated the effects of four typical processing methods: fresh (FB), raw-frozen (RFB), cooked-frozen (FCB), and dried (DB) on the digestive properties of Litopenaeus vannamei muscle protein using an in vitro dynamic gastrointestinal digestion model combined with multi-scale analytical techniques, including SDS-PAGE, endogenous fluorescence spectroscopy, particle size distribution, confocal laser scanning microscopy (CLSM) and peptidomics. The results demonstrated that processing methods significantly altered protein structure, enzymatic resistance, and digestive product characteristics. SDS-PAGE revealed persistent MHC bands in DB samples throughout gastric digestion, whereas FB and RFB exhibited complete degradation. Endogenous fluorescence spectroscopy results indicated that processing inhibit the unfolding of proteins during gastrointestinal digestion. Particle analysis and CLSM confirmed that the DB group still presented large, dense and insoluble residues after gastrointestinal digestion, severe dehydration in dry-heat treatment formed dense and stable protein aggregates that markedly hindered pepsin and trypsin penetration, resulting in the lowest digestion efficiency. Peptidomics further revealed that myosin and actin were the primary peptide sources, with over 80% of cleavage sites located in helix structures. The raw-frozen samples better preserved protein digestibility and nutritional value. These findings provide a theoretical basis for optimizing shrimp processing techniques to enhance protein digestibility and nutritional quality.
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