Related Experiment Video
Updated: Jun 12, 2026

Phosphoproteomic Strategy for Profiling Osmotic Stress Signaling in Arabidopsis
Published on: June 25, 2020
Comprehensive analysis of HB-PHD transcription factors associated with drought and salt stresses in wheat
Zhaosashuang Tian1, Zilin Fan1, Yuyi Ma1
1Yantai Key Laboratory of Molecular Breeding for High-Yield and Stress-Resistant Crops and Efficient Cultivation/College of Horticulture, Ludong University, Yantai, 264000, China.
Background:
HB-PHD transcription factors play important roles in plant growth and development as well as stress response. However, comprehensive analysis of HB-PHD genes was not investigated in wheat.
Results:
In this study, a total of nine TaHB-PHD genes were identified in wheat at the genome-wide level, and their physicochemical properties, evolutionary relationships, gene structures, cis-acting elements, expression patterns, downstream genes, and drought-resistant superior haplotypes were systematically analyzed. Phylogenetic analysis showed that HB-PHD proteins were divided into five subfamilies, and TaHB-PHD genes belonged to subfamilies Ⅰ,Ⅱ, and Ⅴ. The gene structures and conserved motifs of members in the same subfamily were highly conserved. Tissue expression analysis revealed that TaHB-PHD genes had the highest expression levels in roots and the lowest in leaves. Under drought stress, the cultivar-specific expression pattern was observed, all TaHB-PHD genes were down-regulated in drought-tolerant cultivar Giza168, and up-regulated in Chinese Spring and drought-sensitive cultivar Gemmiza10. Under salt stress, TaHB-PHD1-A/B/D and TaHB-PHD3-A/B/D were up-regulated, while the expression of TaHB-PHD2-A/B/D showed no obvious change. Prediction of downstream genes found that TaHB-PHDs could regulate various genes such as ribosomal RNA methyltransferase and histone acetyltransferase to participate in stress responses. Haplotype analysis indicated that TaHB-PHD3-D-Hap I was a drought-resistant superior haplotype.
Conclusions:
This study provides a theoretical basis and candidate genes for the functional verification of wheat TaHB-PHD genes and stress-resistant molecular breeding.
Related Concept Videos
Responses to Salt Stress
Responses to Drought and Flooding
Transcription
Transcription is the process of synthesizing RNA from a DNA sequence by RNA polymerase. It is the first step in producing a protein from a gene sequence. Additionally, many other proteins and regulatory sequences are involved in the proper synthesis of messenger RNA (mRNA). Regulation of transcription is responsible for the differentiation of all the different types of cells and often for the proper cellular response to environmental signals.
Transcription Can Produce Different Kinds...
Other Stress Responses in Bacteria
Adaptations that Reduce Water Loss
Responses to Heat and Cold Stress
