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Updated: Jun 12, 2026

Simultaneous Brightfield, Fluorescence, and Optical Coherence Tomographic Imaging of Contracting Cardiac Trabeculae Ex Vivo
Published on: October 2, 2021
Cross-modal fusion driven 5D imaging system integrating spectral, polarization, and 3D modalities
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Traditional multimodal imaging suffers from registration difficulties and weak information interaction due to the independent acquisition of separate modalities. Based on object-plane multiplexed encoding, this paper proposes a spectral-3D cooperative imaging method that integrates the complementary advantages of object-plane multiplexed encoding imaging (simultaneously acquiring spectral and coarse depth information) and polarization imaging (capturing approximate 3D shape and fine surface texture), thereby developing a new multi-dimensional imaging system capable of jointly acquiring spectral, 3D, and polarization information of the target. Hyperspectral information and coarse depth are obtained through an encoding-decoding mechanism, and a multi-scale gradient consistency sign correction (MS-GCSC) model is designed to use coarse depth as a before guide polarization-based 3D reconstruction. This approach effectively resolves azimuthal ambiguity and enables high-precision depth recovery. The system employs a single camera to synchronously capture polarization and encoded images, avoiding registration challenges and achieving simultaneous 3D-spectral-polarization measurement with an average spectral sampling interval of 3 nm and depth accuracy better than 0.1 mm. Experimental results verify that the proposed method achieves significant improvements in accuracy, stability, and adaptability.
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