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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Updated: Jun 12, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects
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Published on: February 8, 2014

Frequency-diverse structured light for optical gradient sensing using heterodyne interferometry.

J Keith Miller, Arash Shiri, Matthew F Reid

    Optics Express
    |June 11, 2026
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    Summary
    This summary is machine-generated.

    This study presents a novel free-space optical sensing method. It uses a frequency-diverse beamlet array to detect refractive index gradients for non-contact acoustic sensing.

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    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry
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    The Generation of Higher-order Laguerre-Gauss Optical Beams for High-precision Interferometry

    Published on: August 12, 2013

    Area of Science:

    • Photonics
    • Optical Sensing
    • Acoustic Metrology

    Background:

    • Current optical sensing methods often require reference arms or embedded elements.
    • Detecting subtle refractive index changes in free space presents significant challenges.

    Purpose of the Study:

    • To develop a novel free-space optical sensing architecture for direct mapping of refractive index gradients.
    • To establish a compact, reconfigurable platform for remote, non-contact acoustic sensing.

    Main Methods:

    • Utilized a frequency-diverse beamlet array for spatial sampling of the medium.
    • Employed heterodyne modulation to map refractive index gradients into sidebands.
    • Developed an analytic framework and experimentally validated the system using an eight-beamlet array at 532 nm.

    Main Results:

    • Achieved > 80 dB beat-to-sidelobe ratios and near-Pascal acoustic sensitivity.
    • Demonstrated parallel sensing channels on a single detector without a reference arm.
    • Established a general framework for non-contact sensing of refractive index gradients.

    Conclusions:

    • The developed architecture enables efficient, non-contact acoustic sensing with high sensitivity.
    • The platform is reconfigurable and supports extension to higher acoustic bandwidths.
    • Potential applications extend beyond acoustics to turbulence and thermal sensing.