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Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

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In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...

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Measurement of X-ray Beam Coherence along Multiple Directions Using 2-D Checkerboard Phase Grating
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Grating parameter characterization based on the combination of dark field geometry-guidance and model-assisted

Jiale Zhang, Qun Yuan, Xiaoxin Fan

    Optics Express
    |June 11, 2026
    PubMed
    Summary

    This study introduces a new method for precise grating characterization using coherence scanning interferometry (CSI). The technique improves accuracy in measuring grating period and height, even under resolution limitations.

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    Area of Science:

    • Metrology
    • Optical Engineering
    • Surface Science

    Background:

    • Accurate grating characterization is crucial in metrology.
    • Coherence Scanning Interferometry (CSI) faces limitations in measuring small grating periods and heights due to resolution and edge effects.
    • Existing CSI methods struggle with interferometric signal superposition caused by grating edges.

    Purpose of the Study:

    • To develop a novel method for accurate grating characterization under resolution-limited conditions.
    • To overcome the limitations of conventional CSI in determining spatial period and height.
    • To enhance grating metrology without requiring hardware modifications.

    Main Methods:

    • Combines dark field geometry guidance with model-assisted interferometric processing.
    • Utilizes dark field imaging for enhanced subpixel grating-edge localization.
    • Employs Fourier-domain analysis with a Fourier-optics-based signal model to eliminate signal superposition.
    • Reconstructs surface topography using a standard envelope-based CSI algorithm.

    Main Results:

    • Achieved < ±2% error in grating period estimation via simulation.
    • Successfully eliminated redundant interferometric components near step edges.
    • Reduced signed relative height error from 5.6% to 0.3% for a 2 µm period grating.
    • Demonstrated improved grating period determination and height measurement accuracy.

    Conclusions:

    • The proposed method effectively enhances grating characterization accuracy under resolution-limited conditions.
    • Dark field geometry guidance and model-assisted processing are key to overcoming CSI limitations.
    • The technique offers a robust solution for precise metrology of gratings and step heights.