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Related Experiment Video

Updated: Jun 12, 2026

Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection
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Development and Functionalization of Electrolyte-Gated Graphene Field-Effect Transistor for Biomarker Detection

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Nanopore-Based Protein Deceleration and Sensing Using Graphene/Si3N4 Dual Membrane Cavity.

Yubin Cao1, Junzhou He1, Wei Si1

  • 1Jiangsu Key Laboratory for Design and Manufacturing of Precision Medicine Equipment, School of Mechanical Engineering, Southeast University, Nanjing, China.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)
|June 11, 2026
PubMed
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Researchers developed a novel graphene/silicon nitride (Si3N4) dual membrane system to improve nanopore protein sequencing. This system effectively slows peptide translocation, enhancing accuracy and paving the way for high-throughput proteomics.

Area of Science:

  • Nanotechnology
  • Proteomics
  • Biophysics

Background:

  • Nanopore sequencing offers rapid, portable protein analysis but requires improved accuracy.
  • Controlling protein translocation speed is crucial for effective nanopore sensing.
  • Current methods face challenges in balancing speed, accuracy, and signal resolution.

Purpose of the Study:

  • To enhance the accuracy and efficiency of single-molecule protein sequencing using nanopores.
  • To investigate methods for controlling peptide translocation rates within nanopores.
  • To develop a novel dual membrane cavity system for improved nanopore sensing.

Main Methods:

  • Designed a graphene/silicon nitride (Si3N4) dual membrane cavity system.
  • Utilized charge regulation and cavity structure engineering to modulate peptide translocation.
Keywords:
cavity structuredecelerationnanoporeprotein sequencingtranslocation rate

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Last Updated: Jun 12, 2026

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  • Investigated the impact of a Si3N4 toroidal cavity on peptide residence time and signal resolution.

    • Main Results:

    • The Si3N4 toroidal cavity significantly reduced peptide translocation rates.
    • Physical steric hindrance and enhanced van der Waals adsorption were key mechanisms.
    • Prolonged peptide residence time and maintained excellent signal resolution were achieved.

    Conclusions:

    • The developed dual membrane system offers a promising approach for high-resolution, high-throughput protein sequencing.
    • Controlling translocation rates via cavity engineering is vital for advancing nanopore proteomics.
    • This work addresses bandwidth limitations in single-molecule protein analysis.