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Updated: Jun 12, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

Published on: April 12, 2019

FOS3D: A Fluorescence-Enabled Toolkit for Characterizing a Three-dimensional Osteosarcoma Model.

William Humble^1,2,3, Wiktor Zywicki^1,3, Enrico Lucarelli⁴

¹Aikenhead Centre for Medical Discovery (ACMD), St Vincent's Hospital Melbourne, Fitzroy, Victoria, Australia.

Advanced Science (Weinheim, Baden-Wurttemberg, Germany)

|June 11, 2026

Summary

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We developed FOS3D, a novel 3D model for osteosarcoma (OS) research. This fluorescence-enabled system allows non-destructive monitoring and drug screening, improving OS therapeutic development.

Area of Science:

Biomedical Engineering
Oncology
Biomaterials Science

Background:

Osteosarcoma (OS) is an aggressive bone cancer with limited treatment advancements.
Traditional 2D cell cultures do not accurately mimic the complex tumor microenvironment.
Existing 3D models often involve low-throughput and destructive analysis methods.

Purpose of the Study:

To develop a scalable, fluorescence-enabled 3D osteosarcoma model (FOS3D) for enhanced tumor microenvironment analysis.
To enable non-destructive, high-content characterization and drug response profiling of OS.
To facilitate the study of OS adaptations within a physiologically relevant 3D context.

Main Methods:

Fabrication of gelatin methacryloyl hydrogels with tunable stiffness (5-50 kPa) encapsulating GFP-expressing OS cells.

Keywords:

GelMA chemotherapeutics fluorescence in vitro models osteosarcoma tissue engineering

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Last Updated: Jun 12, 2026

Three-Dimensional Bone Extracellular Matrix Model for Osteosarcoma

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Published on: April 12, 2019

Intratibial Osteosarcoma Cell Injection to Generate Orthotopic Osteosarcoma and Lung Metastasis Mouse Models

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Published on: October 28, 2021

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Longitudinal Morphological and Physiological Monitoring of Three-dimensional Tumor Spheroids Using Optical Coherence Tomography

Published on: February 9, 2019

Utilized whole-well fluorescence scanning for non-destructive proliferation assessment.

Integrated light-sheet microscopy for volumetric imaging of tumor organization.

Performed transcriptional profiling and immunohistochemistry to analyze microenvironment-driven adaptations.

Conducted longitudinal monitoring for chemotherapeutic screening.

Main Results:

FOS3D models accurately reflect OS stromal stiffness ranges.
Non-destructive fluorescence scanning correlated well with traditional proliferation assays.
Volumetric imaging revealed dynamic changes in tumor spatial distribution.
Identified microenvironment-driven adaptations in ECM remodeling, stemness, and drug resistance.
Demonstrated successful chemotherapeutic screening of OS cell lines, capturing dose-dependent responses and increased 3D treatment tolerance.

Conclusions:

FOS3D offers a scalable and versatile platform for osteosarcoma research.
The model enables non-destructive, longitudinal monitoring and high-content analysis.
FOS3D facilitates robust drug screening in a physiologically relevant 3D tumor microenvironment.
This toolkit advances the characterization and therapeutic development for osteosarcoma.