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Mouse Models of Cancer Study

Mouse Models of Cancer Study

Mice have long served as models for studying human biology and pathology because of their phylogenetic and physiological similarity with humans. They are also easy to maintain and breed in the laboratory, and hence, many inbred strains are now available for research. Studies on mice have contributed immeasurably to our understanding of cancer biology.
The development of transgenic, knockout, and knock-in mice has led to an exponential increase in their use as model organisms in research,...

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Updated: Jun 12, 2026

Orthotopic Mouse Model of Colorectal Cancer

Orthotopic Mouse Model of Colorectal Cancer

Published on: December 4, 2007

Transplantable Orthotopic Mouse Models for Colorectal Cancer Research: Current Strategies and Future Directions.

Chen Chen^1,2,3, Josefine Schardey³, Tobias S Schiergens³

¹MOA Key Laboratory of Animal Virology, Zhejiang Provincial Engineering Research Center of Animal Biological Products, Zhejiang University Center for Veterinary Sciences, Hangzhou, China.

Visceral Medicine

|June 11, 2026

Summary

Orthotopic mouse models are crucial for studying colorectal cancer (CRC) metastasis and tumor biology. These models better mimic human CRC, aiding research into effective treatments.

Keywords:

Colorectal cancer Murine model Orthotopic model Preclinical models

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Last Updated: Jun 12, 2026

Orthotopic Mouse Model of Colorectal Cancer

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Published on: December 4, 2007

Isolation of Circulating Tumor Cells in an Orthotopic Mouse Model of Colorectal Cancer

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Orthotopic Implantation of Patient-Derived Cancer Cells in Mice Recapitulates Advanced Colorectal Cancer

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Published on: February 10, 2023

Area of Science:

Oncology
Preclinical Research
Animal Models

Background:

Colorectal cancer (CRC) is a major cause of cancer death, with metastatic disease having a poor prognosis.
There is a critical need for preclinical models that accurately reflect human tumor biology.
Orthotopic murine colorectal cancer models offer a more relevant recapitulation of the native tumor microenvironment and metastatic pathways compared to other systems.

Purpose of the Study:

To review transplantable orthotopic colorectal cancer (CRC) mouse models.
To discuss their establishment techniques, applications, and limitations.
To guide researchers in selecting appropriate models for specific biological questions.

Main Methods:

A systematic literature search was conducted in PubMed using relevant keywords.
Commonly used orthotopic implantation methods (open surgical, minimally invasive transanal) were described.
Key design choices and practical considerations were compared, including tumor source, implantation site, and readouts.

Main Results:

Orthotopic models are essential for investigating site-specific tumor-host interactions and metastasis in CRC.
Model selection depends on the biological question, immune context, and technical feasibility.
Various approaches exist for establishing these models, each with specific advantages and limitations.

Conclusions:

Transplantable orthotopic models are indispensable for studying colorectal cancer (CRC) metastasis.
Model selection should be informed by the research question, immune context, and feasibility.
Further refinement and standardization, including immune and microenvironmental components, will enhance translational relevance.