Related Experiment Video
Updated: Jun 12, 2026
Establishment and Evaluation of a Sheep Model of Full-thickness Osteochondral Defect
Published on: April 14, 2026
Engineered Chondrocalcin From Pichia pastoris: A Dual-Functional Biomaterial for Cartilage Regeneration and
Wensha Zhu1,2,3, Zilong Zhao1,2,3, Weigang Yuwen2
1School of Chemical Engineering, Engineering Research Center of Western Resource Innovation Medicine Green Manufacturing, Ministry of Education, Northwest University, Xi'an, China.
Abstract:
Chondrocalcin (CCN), the C-terminal propeptide of type II procollagen and a key regulatory peptide in collagen assembly, is abundantly expressed in cartilage and implicated in bone-cartilage interface disorders. We engineered a Pichia pastoris strain via genetic modification to produce recombinant chondrocalcin (rCCN), which was isolated, purified, and characterized. Functional assays revealed that rCCN promotes human bone marrow mesenchymal stem cell (hBMSC) differentiation into chondrocytes. Mechanistically, rCCN at 0.3 mg/mL promoted hBMSC chondrogenesis via SOX9/COLII/aggrecan upregulation (2.84-, 7.94-, 9.13-fold) while suppressing fibrotic COL I. In rheumatoid arthritis synoviocytes (HFLS-RA), rCCN reprogrammed the inflammatory microenvironment via the upregulation of COLII/IL-4/TGF-β1 upregulating (0.69-, 4.01-, 1.18-fold), and the downregulating TNF-α, IL-1β, and IL-6. These findings highlight rCCN's dual therapeutic potential as a biomaterial for cartilage repair and inflammatory modulation.
