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Spindle Assembly

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Spindle assembly occurs through three, often coexisting, pathways – the centrosome-mediated pathway, the chromatin-mediated pathway, and the microtubule-mediated pathway – collectively contributing to form a robust spindle apparatus.
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Centrosome Duplication

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Related Experiment Video

Updated: Jun 12, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

Published on: December 20, 2014

Centriolar satellites assemble via a hierarchical pathway driven by PCM1 multimerization.

Efe Begar¹, Ece Seyrek¹, Selin Yilmaz-Karaoglu¹

¹Department of Molecular Biology and Genetics, Koç University, Istanbul, Turkey.

The Journal of Cell Biology

|June 11, 2026

Summary

Centriolar satellites (CS) assemble through a hierarchical pathway starting with pericentriolar material-1 (PCM1) scaffolds. This study defines the molecular basis of CS biogenesis and its links to disease.

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Last Updated: Jun 12, 2026

Quantitative Immunofluorescence Assay to Measure the Variation in Protein Levels at Centrosomes

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Immunofluorescence Analysis of Endogenous and Exogenous Centromere-kinetochore Proteins

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Area of Science:

Cell Biology
Molecular Biology
Organelle Biology

Background:

Centriolar satellites (CS) are vital membraneless organelles implicated in various cellular functions and diseases.
The molecular mechanisms governing CS assembly and regulation are not well understood.

Purpose of the Study:

To elucidate the molecular principles of centriolar satellite biogenesis and regulation.
To develop novel assays for quantifying CS properties during their lifecycle.

Main Methods:

Development of cellular and in vitro biogenesis assays for spatiotemporal quantification of CS.
High-resolution imaging to analyze the subdomains and dynamics of PCM1 and its clients.
Investigation of the role of pericentriolar material-1 (PCM1) scaffold formation and multimerization.

Main Results:

CS assembly follows a hierarchical pathway initiated by PCM1 scaffold formation and subsequent client recruitment.
PCM1 intrinsically forms granules via multimerization, a process influenced by the cytoskeleton.
Disruption of PCM1 multimerization negatively impacts ciliary signaling and mitotic progression.

Conclusions:

This study defines the molecular basis of centriolar satellite biogenesis, providing essential tools for further research.
The findings offer a framework for understanding how CS dysfunction contributes to developmental and neuronal diseases.
The principles of CS assembly may be applicable to other membraneless organelles, explaining their specificity and plasticity.