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Sinomenine Regulates PSMB9 to Mediate Therapeutic Effects in Rheumatoid Arthritis
Cui Zhang1, Chonkit Lio2,3, Nana Li1
1Department of Basic Medicine, Department of Pharmacy, Key Laboratory on the Property & Effect of Chinese Medicine (Ethnic Medicine), Guizhou Genuine Herbs Center of Consistency of Utility, Guizhou Key Laboratory of Miao Medicine, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China.
Researchers identified PSMB9 as a key gene in rheumatoid arthritis (RA) and found it interacts with sinomenine (SIN). This discovery offers potential diagnostic biomarkers and therapeutic targets for RA, highlighting SIN
Area of Science:
- Immunology
- Molecular Biology
- Pharmacology
Background:
- Rheumatoid arthritis (RA) is a systemic autoimmune disease causing chronic inflammation and joint damage.
- The molecular mechanisms and diagnostic biomarkers for RA are not fully understood.
Purpose of the Study:
- Identify potential diagnostic biomarkers for RA.
- Investigate the association between biomarkers and immune infiltration in RA.
- Explore the therapeutic potential of sinomenine (SIN) in RA.
Main Methods:
- Integrated differentially expressed genes (DEGs) and weighted gene co-expression network analysis (WGCNA).
- Performed gene set enrichment analysis (GSEA) and immune infiltration analysis.
- Utilized single-cell RNA sequencing, cellular thermal shift assay (CETSA), and Western blot.
Main Results:
- Identified PSMB9 as a hub gene significantly associated with RA.
- Found a strong link between RA and macrophage infiltration, with PSMB9 highly expressed in macrophages and synovial cells.
- Validated direct binding of sinomenine (SIN) to PSMB9, leading to decreased PSMB9 protein expression.
Conclusions:
- PSMB9 shows potential as a diagnostic biomarker and therapeutic target for RA.
- Sinomenine (SIN) may exert anti-inflammatory effects in RA by regulating PSMB9.
- This study provides insights into RA mechanisms and identifies a novel molecular target for SIN.
