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Updated: Jun 12, 2026

Visualization of ATP Synthase Dimers in Mitochondria by Electron Cryo-tomography
Published on: September 14, 2014
ATP Synthase Subunit b Binds Cry2Ab and Mediates Its Toxicity in Grapholita molesta
Xiaoyan Yang1, Yanshen Fu1, Jiacheng Ye1
1Key Laboratory of Plant Protection Resources and Pest Management of Ministry of Education, Key Laboratory of Integrated Pest Management on Crops in Northwestern Loess Plateau of Ministry of Agriculture and Rural Affairs, College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Abstract:
The oriental fruit moth (Grapholita molesta), a worldwide fruit tree pest, can be effectively controlled by Bacillus thuringiensis (Bt), with the insect peritrophic membrane (PM) serving as a key target for such control. This study investigates the role of the key PM protein ATP synthase subunit b (GmolATPs-b) in the molecular mechanism of Cry2Ab toxin toxicity. GmolATPs-b was found to be highly expressed in fourth larval midguts, and its expression is downregulated upon Cry2Ab exposure. Ligand blotting, competitive experiments, and molecular docking confirmed specific binding between GmolATPs-b and activated Cry2Ab. Heterologous expression in Sf9 cells enhanced susceptibility to Cry2Ab, whereas larval RNAi knockdown reduced it. In vivo assays showed that cofeeding purified GmolATPs-b with Cry2Ab increased larval mortality. This study demonstrates that GmolATPs-b is a functional target of Cry2Ab, binding specifically to the toxin and enhancing its insecticidal effect, thus establishing a molecular basis for novel biopesticides against G. molesta.
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