Electrostatic Microsphere Intestinal Aging Model and Traditional Chinese Medicine Antiaging Mechanism Study

Intestinal organoids mimic the morphological, differentiation, secretory, and absorptive physiological processes of the gut, making them an ideal model for studying antiaging mechanisms and screening bioactive compounds. However, traditional intestinal organoid culture methods have limitations in controlling their size and growth. In this study, we employed electrostatic spraying technology to successfully construct induced pluripotent stem cell microspheres, which were further induced into intestinal organoids with improved controllability. Based on this, we established an aging model of intestinal organoids to investigate the antiaging mechanisms of bioactive components derived from traditional Chinese medicine (TCM). Using a lipopolysaccharide-induced intestinal aging model, we observed elevated levels of aging markers (P16 and P21) and proinflammatory cytokines (IL-1β, TNF-α, and MCP-1), confirming the successful establishment of the aging model. Among the 10 TCM components screened using this model, myricetin was found to effectively suppress intestinal aging by inhibiting NF-κB activation and alleviating inflammation. Transcriptomic analysis revealed that the antiaging mechanism of myricetin involves the modulation of MAPK, PI3K/Akt, and NF-κB signaling pathways. This study provides an approach for the screening of TCM-derived antiaging compounds and offers important insights into the controllable preparation of three-dimensional organoids.