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Published on: May 3, 2024
Label-Free SERS Detection of Molecular Stress Response in the Golgi Apparatus Induced by Pulsed Electrical
Jiao Kong1,2, Xingkai Ju1,2, Guohua Qi3
1Key Laboratory of Electroanalytical Chemistry Changchun Institute of Applied Chemistry, Chinese Academy of Sciences, Changchun 130022, Jilin, P. R. China.
Abstract:
Regulating neuronal cell differentiation in a controllable manner is crucial for the treatment of nervous system regeneration. In this study, we present a method utilizing pulsed electrical stimulation (PES) to accelerate the differentiation of SH-SY5Y cells and investigate the molecular stress response of the Golgi apparatus (GA) through SERS spectra. Compared with traditional drug stimulation (10 days), this simple PES approach within 2 days achieves a 5-fold increase in the rate of generating SH-SY5Y-derived neurons. PES-treated neuron cells showed an increased level of mature neuronal marker Tuj1 and increased expression of related genes Tubb3 and Eno2. We found that PES induces SH-SY5Y cell differentiation by regulating the expression of MAPK/ERK and calcium signaling pathway proteins. The GA-related changes in this pathway are shown by SERS spectra. In situ SERS shows that the content levels rise while lipid levels drop with prolonged differentiation. This work offers a new strategy for SH-SY5Y differentiation and insights into the GA molecular stress response.
