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Updated: Jun 13, 2026
Chemiluminescence-based Assays for Detection of Nitric Oxide and its Derivatives from Autoxidation and Nitrosated Compounds
Published on: February 16, 2022
A Multiplex Immunochromatographic Assay for the Simultaneous Detection of Nitroxynil and Oxyclozanide in Food and
Qingqing Liu1,2, Liqiang Liu1,2, Yangmei Liu3
1State Key Laboratory of Food Science and Resources, Jiangnan University, Wuxi, Jiangsu 214122, China.
Abstract:
The widely used deworming drugs nitroxynil (NIT) and oxyclozanide (OXY) pose potential risks to the ecological environment and human health, necessitating a sensitive and rapid detection method. In this study, two novel haptens targeting OXY and NIT, respectively, were designed and synthesized, with their structural validity evaluated by computer simulation. Through animal immunization and cell fusion, monoclonal antibodies (OXY-1A4 and NIT-2F12) were obtained, with half-maximal inhibitory concentrations of 1.13 ng/mL and 0.23 ng/mL, respectively. Based on this, a multiplex gold nanoparticle-based immunochromatographic assay (multi-AuNPs-ICA) was established for simultaneous detection of OXY and NIT in wastewater, milk, and beef. Visual and calculated limits of detection ranged from 2 to 20 ng/mL and 0.37-7.16 ng/mL, respectively. Spiked recovery rates in wastewater, milk, and beef samples were 102.6%-110.5%, 90.4%-115.6%, and 86.9%-117.7%, respectively. These were highly consistent with instrumental methods, confirming the reliability of multi-AuNPs-ICA and its significant potential for on-site rapid screening.
