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Updated: Jun 13, 2026
Technique of Subnormothermic Ex Vivo Liver Perfusion for the Storage, Assessment, and Repair of Marginal Liver Grafts
Published on: August 13, 2014
Impact of Oxygenated Hypothermic Machine Perfusion for Pig Small Intestine
Mio Takashima1, Satoshi Muto1, Daisuke Ishii2
1Tokyo Metropolitan University, Mechanical Systems Engineering, Tokyo, Japan.
Background:
Intestinal transplantation (IT) is an important treatment for end-stage intestinal failure. However, the survival rate is lower than that of other organs. Machine perfusion (MP) is used in other organs and provides better preservation than simple cold storage (SCS). This study compared SCS and hypothermic machine perfusion (HMP) in the small intestine of a pig. We aim to compare the effectiveness of organ preservation and evaluate the motor function by measuring peristalsis.
Method:
Pig small intestines were extracted and preserved in the SCS group (SCS 6 hours) or HMP group (SCS 4 hours + HMP 2 hours), and then reperfusion was performed for 2 hours as an isolated reperfusion model (IRM). Pathological evaluation was based on the Swerdlow Classification. During reperfusion, peristalsis was measured using the digital image correlation method.
Result:
At 120 minutes during IRM, vascular resistance was 1.02±0.57 vs 0.31±0.05 mm Hg/mL/min (SCS vs HMP, p < .05). Oxygen consumption was 1.2±0.8 vs 2.3±0.3 mg/min (SCS vs HMP, p < .05). The peristalsis was 0.22±0.15 vs 0.47±0.15 mm/s (SCS vs HMP, p < .05) at 90 minutes of IRM. The mucosal tissue after preservation was Grade 1 in both SCS group cases and Grade 0 and Grade 2 in each HMP group case. After reperfusion, mucosal necrosis was confirmed in 2 SCS cases and 1 HMP case, while the mucosa was preserved in one HMP case.
Conclusion:
HMP provides better preservation of vascular resistance, oxygen consumption, and peristalsis in the small intestine after ex vivo reperfusion than SCS.
