Related Experiment Video
Updated: Jun 13, 2026
07:16
Resin-Assisted Capture Coupled with Isobaric Tandem Mass Tag Labeling for Multiplexed Quantification of Protein Thiol Oxidation
Published on: June 21, 2021
Bridging oxidative post-translational modifications to biological meaning
Maolin Ge1, Magdy Gohar2, Liron Bar-Peled3
1Krantz Family Center for Cancer Research, Massachusetts General Hospital Cancer Center, Charlestown, MA 02129, USA; Department of Medicine, Harvard Medical School, Boston, MA 02114, USA.
Trends in Cell Biology
|June 11, 2026
Summary
Identifying reactive oxygen species (ROS) targets requires more than just oxidation levels. Integrating functional genomics with proteomics helps uncover regulatory redox modifications and signaling pathways.
Area of Science:
- Biochemistry
- Molecular Biology
- Cellular Signaling
Background:
- Reactive oxygen species (ROS) are crucial signaling molecules involved in cellular processes.
- Proteomics has identified numerous ROS targets, highlighting redox modifications as key post-translational regulators.
- Distinguishing regulatory ROS modifications from damaging ones is a significant challenge.
Purpose of the Study:
- To evaluate the strategy of prioritizing ROS targets based solely on oxidation levels.
- To advocate for integrating functional genomics with proteomics for robust discovery.
- To accelerate the identification of novel ROS-mediated signaling pathways.
Main Methods:
- Review of current proteomics techniques for identifying ROS targets.
- Discussion of the limitations of relying solely on oxidation levels.
- Proposal for a combined functional genomics and proteomics approach.
Main Results:
- Prioritizing ROS targets by oxidation level alone may overlook functionally relevant modifications.
- Functional genomics provides an orthogonal method to validate redox-based protein regulation.
- Integrating these approaches enhances the discovery of metabolite-modified signaling.
Conclusions:
- A combined proteomics and functional genomics strategy is essential for understanding redox regulation.
- This integrated approach overcomes limitations in identifying functional ROS targets.
- It facilitates a deeper understanding of cellular signaling pathways regulated by ROS and other metabolites.
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