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Related Concept Videos

Raman Spectroscopy Instrumentation: Overview01:26

Raman Spectroscopy Instrumentation: Overview

A conventional Raman spectrophotometer includes a laser source, a sample holding system, a wavelength selector, and a detector.
The monochromatic laser source, typically using visible or near-infrared radiation, generates a highly focused beam of light. This light interacts with the molecules of the sample, scattering some of the light. Liquid and gaseous samples are usually tested in ordinary glass capillaries, while solids can be analyzed as powders packed in capillaries or as potassium...

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Automated high-throughput Raman detection of fluidic samples: measurement setup and methods preventing air bubble

Jingyi Chen1,2, Jiarui Wang1, Elmar Schuck1

  • 1Boehringer Ingelheim Pharma GmbH/Co. KG, Biberach an Der Riss, Germany.

Analytical and Bioanalytical Chemistry
|June 11, 2026
PubMed
Summary
This summary is machine-generated.

A new automated Raman spectroscopy setup allows high-throughput analysis of low-volume liquid samples (300 µL). Optimized methods effectively reduce air bubbles, enhancing spectral data quality for biopharmaceutical processing.

Keywords:
Air-free detectionAutomationBubble interferenceFlow-cell injectionLiquid handling stationRaman spectroscopy

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Area of Science:

  • Analytical Chemistry
  • Biopharmaceutical Analysis

Background:

  • Raman spectroscopy is valuable for biopharmaceutical downstream processing.
  • Existing flow cell methods are limited for low-volume samples (<1 mL).
  • In-line monitoring struggles with inaccessible sample streams.

Purpose of the Study:

  • To develop an automated Raman spectroscopy setup for micro-volume liquid samples.
  • To implement methods for mitigating air bubble interference in Raman measurements.
  • To enable high-throughput analysis of small sample volumes.

Main Methods:

  • Integration of a Tornado Micro Flow Cell with a Tecan liquid handling system via a custom adapter.
  • Development of air bubble mitigation strategies: adapted residence time, three-step purging/cleaning, and vertical/upflow orientation.
  • Automated sample delivery and Raman spectral acquisition for liquid samples.

Main Results:

  • Successful automated Raman measurements with injection volumes as low as 300 µL.
  • Demonstrated reduction in air bubble presence and improved spectral robustness.
  • Validation of the setup and methods for high-throughput analysis of small fluidic samples.

Conclusions:

  • The novel setup and integrated methods enable automated, high-throughput Raman analysis of micro-volume liquid samples.
  • Effective air bubble management significantly improves spectral quality and reliability.
  • This approach expands the applicability of Raman spectroscopy in biopharmaceutical development and quality control.