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Updated: Jun 13, 2026
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Published on: April 5, 2022
Pyridoxal-Based Selective Chemo Sensor for Colorimetric and Fluorescent Detection of Copper Ions and Imaging in Live
Kisan Pawar1, Mareechika Gaddam1, Sujit Suryawanshi1
1Department of Medicinal Chemistry, National Institute of Pharmaceutical Education and Research, Raebareli, India.
Abstract:
A novel pyridoxal-based chemo sensor (PDBI) was synthesized, characterized and demonstrated for the detection of Cu2+ ions. The sensing ability of the chemo sensor was assessed in a 10% DMSO-HEPES pH 7.4 buffer medium using both fluorescence and colorimetric methods. PDBI exhibited greater binding affinity and selectivity for Cu2+ by showing a fluorescence ON-OFF phenomenon and colorimetric response from yellow to brown. The limit of detection of PDBI to Cu2+ was calculated to be 28 nM. The stoichiometric ratio of Cu2+ to PDBI in the complex is 1:1, as indicated by the job's plot. The chelation mechanism was supported by partial 1H-NMR, HRMS and quantum chemical calculations. PDBI was converted into portable paper strips for the detection of Cu2+ in real samples, and also enabled the detection of copper in live cells.
