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Related Concept Videos

Regulation of Food Intake01:30

Regulation of Food Intake

Short-term regulation of food intake primarily involves neural signals from the gastrointestinal (GI) tract, blood nutrient levels, and GI tract hormones. Communication between the gut and brain via vagal nerve fibers plays a significant role in evaluating the contents of the gut. Clinical studies have shown that protein ingestion produces a more prolonged response in these nerve fibers compared to an equivalent amount of glucose. Additionally, the activation of stretch receptors caused by GI...

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Related Experiment Video

Updated: Jun 13, 2026

An Experimental Model of Diet-Induced Metabolic Syndrome in Rabbit: Methodological Considerations, Development, and Assessment
10:31

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Published on: April 20, 2018

M6A Regulates Intramuscular Fat Deposition in Rabbits Through LPL/3-Methyl-L-Histidine/Pathways.

Gang Luo1, Jihao Le1, Xiaoming Mao1

  • 1College of Animal Science, Fujian Agriculture and Forestry University, Fuzhou 350000, China.

Animals : an Open Access Journal From MDPI
|June 12, 2026
PubMed
Summary
This summary is machine-generated.

N6-methyladenosine (m6A) impacts rabbit meat flavor by regulating intramuscular fat. METTL3 inhibits LPL expression via m6A, influencing fat deposition through specific metabolic pathways for better meat quality.

Keywords:
IMFLPLM6AMETTL3Rex rabbits

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Area of Science:

  • Animal Science
  • Molecular Biology
  • Epigenetics

Background:

  • Rabbit meat flavor is crucial for industry development, with intramuscular fat being a key determinant.
  • N6-methyladenosine (m6A) is an epigenetic modification influencing various physiological processes in animals.

Purpose of the Study:

  • To elucidate the regulatory mechanism of m6A on intramuscular fat deposition in rabbits.
  • To investigate the role of m6A in modulating the expression of the LPL gene and its downstream effects.

Main Methods:

  • Quantitative real-time PCR (qRT-PCR) and m6A-qPCR were employed to identify target genes and methylation levels.
  • Adeno-associated virus (AAV) vectors were used for gene overexpression.
  • RNA interference was utilized to explore m6A regulation of the LPL gene at the cellular level.

Main Results:

  • METTL3 was found to inhibit LPL expression through m6A modification, recognized by YTHDF2 in adipocytes and muscles.
  • LPL promotes adipocyte differentiation and intramuscular fat deposition.
  • LPL regulates intramuscular fat deposition via L-Glutamine/multiple pathways and 3-Methyl-L-histidine.

Conclusions:

  • m6A modification influences LPL gene expression in rabbits, thereby regulating intramuscular fat deposition.
  • This regulation occurs through L-Glutamine/multiple pathways and 3-Methyl-L-histidine.
  • The findings provide a molecular basis for developing strategies to cultivate high-quality rabbit meat.