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Updated: Jun 13, 2026
04:40
The Cultivation, Growth, and Viability of Lactic Acid Bacteria: A Quality Control Perspective
Published on: June 16, 2022
Comparative Study on Biological Characteristics and Functions of Three Porcine-Derived Lactic Acid Bacteria
Miao Yu1,2, Yaojun Li2, Bing Yu1
1Animal Nutrition Institute, Sichuan Agricultural University, Chengdu 611130, China.
Animals : an Open Access Journal From MDPI
|June 12, 2026
Summary
This study evaluated three lactic acid bacteria strains for swine production. Lactobacillus salivarius MRS004 showed the best in vitro performance, while Lactobacillus plantarum MRS002 demonstrated broad adaptability and Lactobacillus amylovorus MRS003 is suitable for high-starch feed.
Area of Science:
- Microbiology
- Animal Science
- Genomics
Background:
- Lactic acid bacteria (LAB) are crucial for animal gut health and production efficiency.
- Screening high-quality LAB strains is essential for developing effective probiotics in swine production.
- Porcine-derived LAB offer potential for improved gut microbiome modulation in pigs.
Purpose of the Study:
- To compare the in vitro characteristics of three porcine-derived lactic acid bacteria strains: Lactobacillus plantarum MRS002, Lactobacillus amylovorus MRS003, and Lactobacillus salivarius MRS004.
- To evaluate their growth performance, acid production, acid and bile salt tolerance, and genomic features for swine production applications.
- To identify superior LAB strains for probiotic development in the swine industry.
Main Methods:
- In vitro assays were conducted to assess growth kinetics, acid production (pH monitoring), and tolerance to low pH (pH 2.0) and bile salts (0.3%).
- Genome annotation was performed to analyze metabolic pathways and stress-resistance gene networks.
- Statistical analysis included one-way ANOVA and Tukey's multiple comparison test (p < 0.05) with three biological replicates.
Main Results:
- All three LAB strains exhibited anaerobic growth. Lactobacillus amylovorus MRS003 showed a longer growth cycle and higher biomass.
- Lactobacillus plantarum MRS002 and Lactobacillus salivarius MRS004 demonstrated faster growth, higher acid production (pH 4.2-4.3 at 24h), and superior survival under acid and bile salt stress.
- Genomic analysis revealed Lactobacillus plantarum MRS002 has extensive metabolic and stress-resistance genes, Lactobacillus amylovorus MRS003 is enriched in starch-degradation pathways, and Lactobacillus salivarius MRS004 possesses unique aromatic amino acid metabolism pathways.
Conclusions:
- Lactobacillus salivarius MRS004 exhibits favorable phenotypic characteristics for probiotic use.
- Lactobacillus plantarum MRS002 offers broad adaptability, making it a versatile candidate.
- Lactobacillus amylovorus MRS003 is well-suited for high-starch feed fermentation, providing a basis for targeted probiotic product development.
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