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Modeling Soluble Protein Fractionation in Feedstuffs in Equine Rations Using Crude Protein and Fiber Composition.
Ryon W Springer1, James P Muir2,3, Kimberly B Wellmann1
1Department of Animal Science, Tarleton State University, Stephenville, TX 76402, USA.
Animals : an Open Access Journal From MDPI
|June 12, 2026
Summary
Accurate equine protein requirements depend on digestible protein fractions, not just crude protein. New models predict neutral detergent soluble crude protein (NDSCP) and pre-cecal digestible crude protein (pcdCP) using feedstuff crude protein and fiber content.
Area of Science:
- Animal Nutrition
- Equine Science
- Feedstuff Analysis
Background:
- Current equine protein requirements rely on crude protein (CP), overlooking its digestibility.
- Protein distribution within feedstuffs impacts foregut digestion and nutrient availability.
- Neutral detergent soluble crude protein (NDSCP) represents the readily digestible protein fraction crucial for equine needs.
Purpose of the Study:
- To develop linear regression models for predicting NDSCP and pre-cecal digestible crude protein (pcdCP).
- To utilize feedstuff crude protein (CP) and fiber components as predictors in these models.
- To enhance the accuracy of equine dietary protein assessment.
Main Methods:
- Data sourced from the 2023 Dairy One© Feedstuff Composition Library.
- Feedstuffs with n ≥ 50 analyses for CP, NDF, ADF, and ADL were selected and categorized.
- Multiple linear regression analysis was performed using R Statistical Program©, with model selection based on AICc, AICcWt, and Adjusted R².
Main Results:
- The model incorporating CP and neutral detergent fiber (NDF) provided the highest predictive accuracy for both NDSCP and pcdCP (AICcWt = 0.95; Adjusted R² = 0.9868 for NDSCP, 0.9867 for pcdCP).
- These findings indicate that CP and NDF effectively predict protein digestibility.
- The developed models demonstrated high precision in predicting available protein.
Conclusions:
- Linear models using CP and NDF accurately predict NDSCP and pcdCP in feedstuffs.
- Incorporating fiber fractionation improves the prediction of available dietary protein for horses.
- This approach offers a more precise method for formulating equine diets to meet protein requirements.

