Related Experiment Video
Updated: Jun 13, 2026
11:48
Isolation Protocol of Mouse Monocyte-derived Dendritic Cells and Their Subsequent In Vitro Activation with Tumor Immune Complexes
Published on: May 31, 2018
Melatonin and Indole-3-Propionic Acid Reduce Surface FcγRIII/CD16-Related Parameters in Porcine Peripheral Blood
Przemysław W Śliwka1, Jan Stępniak1, Małgorzata Karbownik-Lewińska1,2
1Department of Endocrinology and Metabolic Diseases, Medical University of Lodz, 281/289 Rzgowska St., 93-338 Lodz, Poland.
International Journal of Molecular Sciences
|June 12, 2026
Summary
Melatonin and indole-3-propionic acid (IPA) reduce Fc gamma receptor III (CD16) expression on porcine immune cells. These indole compounds show potential immunomodulatory activity in vitro.
Area of Science:
- Immunology
- Molecular Biology
Background:
- Fc gamma receptor III (CD16) is crucial for IgG-mediated immunity, expressed on monocytes, macrophages, and NK cells.
- Altered CD16 expression is linked to inflammatory and autoimmune diseases, including thyroid-associated immune issues.
Purpose of the Study:
- To investigate the in vitro effects of melatonin and indole-3-propionic acid (IPA) on FcγRIII/CD16 expression in porcine peripheral blood mononuclear cells (PBMCs).
Main Methods:
- Porcine PBMCs were cultured alone or with autologous thyroid follicular cells.
- Cells were treated with 50 µM melatonin or IPA and analyzed by flow cytometry at 0, 24, and 48 hours.
- Assessed parameters included the percentage of CD45+CD16+ cells and CD16 mean fluorescence intensity.
Main Results:
- Untreated PBMCs showed a time-dependent decrease in CD16 expression.
- Melatonin and IPA treatments further enhanced this decrease compared to controls.
- Co-culture with thyroid follicular cells did not significantly alter CD16-related parameters.
Conclusions:
- Melatonin and IPA may modulate the CD16 phenotype on porcine leukocytes in vitro.
- Indole-derived compounds show potential immunomodulatory effects on CD16-expressing leukocytes.
- Further research in advanced models is warranted to confirm these preliminary findings.
