Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Renewal of Intestinal Stem Cells

Renewal of Intestinal Stem Cells

The intestinal epithelial lining rapidly renews every 4 to 5 days. The renewal is facilitated by intestinal stem cells (ISCs) located at the base of the crypt– a gland located at the bottom of each villus. ISCs divide asymmetrically to form new stem cells and progenitor daughter cells. The daughter cells are called transit-amplifying (TA) cells which move upwards along the crypt and either differentiate into absorptive cells– the enterocytes or secretory cells– including the goblet,...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Breaking the Diffraction-Encoding Limit for High-Capacity Meta-Holography via Multiorder Decoupling.

ACS nano·2026

Same author

AM-PM conversion in the residual phase noise of two-point electro-optical frequency division.

Optics letters·2026

Same author

Stimulated inter-modal Brillouin scattering on suspended anti-resonant acoustic waveguides.

Optics letters·2026

Same author

Cancer-Associated Fibroblasts Promote Tumor Immunosuppression in Hepatocellular Carcinoma via the NNMT-ANGPTL4 Axis.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same author

Efficacy analysis of small-incision in situ decompression under ultrasound combined with shear-wave elastography in the treatment of ulnar neuropathy at the elbow.

International orthopaedics·2026

Same author

Low-spin state design of highly active diatomic catalysts for oxygen reduction reaction.

National science review·2026

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

Published on: November 3, 2014

Integrated Single-Cell and Spatial Transcriptomic Analysis Identifies Putative Metabolic Crosstalk Between SPP1+ TAMs

Yu Xue^1,2, Guangsong Tang^2,3, Xinglong Li^2,4

¹School of Life Sciences, Faculty of Medicine, Tianjin University, Tianjin 300072, China.

|June 12, 2026

Summary

Colorectal cancer (CRC) involves metabolic reprogramming in malignant epithelial cells and tumor-associated macrophages (TAMs). This study reveals coordinated metabolic programs and bidirectional communication, leading to a new prognostic model for CRC patient stratification.

Keywords:

colorectal cancer metabolic crosstalk metabolic reprogramming prognostic model scRNA-seq spatial transcriptomics

More Related Videos

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Published on: April 29, 2014

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Related Experiment Videos

Last Updated: Jun 13, 2026

Real Time Analysis of Metabolic Profile in Ex Vivo Mouse Intestinal Crypt Organoid Cultures

Published on: November 3, 2014

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Molecular Profiling of the Invasive Tumor Microenvironment in a 3-Dimensional Model of Colorectal Cancer Cells and Ex vivo Fibroblasts

Published on: April 29, 2014

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Multiplexed Analysis of Retinal Gene Expression and Chromatin Accessibility Using scRNA-Seq and scATAC-Seq

Published on: March 12, 2021

Area of Science:

Oncology
Metabolic Biology
Immunology

Background:

Colorectal cancer (CRC) progression is linked to tumor metabolic reprogramming and an immunosuppressive microenvironment.
Coordinated metabolic interactions between malignant epithelial and immune cells in CRC are not well understood.

Purpose of the Study:

To characterize metabolic crosstalk in colorectal cancer.
To validate spatial organization of metabolic interactions.
To develop a metabolism-based prognostic model for CRC.

Main Methods:

Integrated six colorectal cancer single-cell RNA sequencing datasets.
Utilized spatial transcriptomics to assess regional co-enrichment.
Analyzed bulk transcriptomic cohorts and targeted metabolomics data.
Employed elastic net modeling for prognostic signature development.

Main Results:

Identified metabolically reprogrammed SLC6A20+ epithelial cells and immunosuppressive SPP1+ tumor-associated macrophage (TAM) subsets in CRC.
Both subsets showed elevated glycolysis, vitamin B6, and aromatic amino acid metabolism.
Spatial transcriptomics confirmed regional co-enrichment of these cell subsets and shared metabolic activities.
A 14-gene signature based on metabolic features predicted high-risk CRC subtypes.

Conclusions:

SLC6A20+ epithelial cells and SPP1+ TAMs exhibit coordinated metabolic programs and bidirectional communication in CRC.
These findings offer potential biomarkers for CRC.
A metabolism-based prognostic model can stratify CRC patients effectively.