Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 13, 2026

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

IDH1-Associated m6A Methylation Is Linked to Transcriptomic Heterogeneity in Glioma.

Syeda Maheen Batool1, Hanna Lee1, Koushik Muralidharan1

  • 1Department of Neurosurgery, Massachusetts General Hospital, Harvard Medical School, Boston, MA 02114, USA.

Cancers
|June 12, 2026
PubMed
Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Tumor infiltrating lymphocytes in glioblastoma: immunobiology and translational implications.

NPJ precision oncology·2026
Same author

Automating neoantigen selection for personalized cancer vaccine design.

medRxiv : the preprint server for health sciences·2026
Same author

A modular and flexible pipeline for intraoperative electrode reconstruction and localization in patients with brain lesions.

Frontiers in neural circuits·2026
Same author

Clinical validation of an HPV whole-genome sequencing assay for MRD detection in patients with HPV+ head and neck cancer treated with surgery.

Science translational medicine·2026
Same author

Long-read transcriptomic profiling of a glioma cell line following m6A regulator perturbations.

Biochemistry and biophysics reports·2026
Same author

Adjuvant personalized multivalent neoantigen DNA vaccination for MGMT unmethylated glioblastoma: a phase 1 trial.

Nature cancer·2026

This study reveals subtype-specific N6-methyladenosine (m6A) patterns in glioma, highlighting isoform diversity crucial for understanding tumor biology and potential therapeutic targets.

Area of Science:

  • Epigenetics
  • Cancer Biology
  • Transcriptomics

Background:

  • N6-methyladenosine (m6A) epigenetic modifications are vital for glioma biology.
  • Current short-read sequencing methods limit transcriptome-wide m6A mapping at isoform resolution in glioma subtypes.
  • Alternative splicing is prevalent in glioma, necessitating isoform-level m6A analysis.

Purpose of the Study:

  • To map the m6A epigenetic landscape at isoform resolution across specific glioma subtypes.
  • To investigate subtype-specific patterns of m6A modifications and their relationship with gene expression and isoform usage.
  • To explore the potential of isoform-level m6A analysis for identifying glioma biomarkers and therapeutic targets.

Main Methods:

  • Direct RNA nanopore sequencing and transcriptome-wide m6A analysis were performed on 14 glioma tumor tissues.
Keywords:
IDH1N6-methyladenosineepitranscriptomicsgliomaisoformsnanopore sequencing

Related Experiment Videos

Last Updated: Jun 13, 2026

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV
14:40

Exploring m6A and m5C Epitranscriptomes upon Viral Infection: an Example with HIV

Published on: March 5, 2022

  • Included were IDH1-mutant astrocytoma, oligodendroglioma, and IDH1 wild-type glioblastoma subtypes.
  • m6A sites were computationally predicted using the m6Anet deep learning framework with high-confidence calls.

    • Main Results:

    • IDH1-mutant gliomas exhibited a higher burden of m6A modifications compared to IDH1 wild-type glioblastoma.
    • Subtype-specific m6A patterns were identified, often independent of gene-level expression changes, indicating a post-transcriptional regulatory layer.
    • Integration of gene expression, isoform usage, and m6A status revealed variations in isoform composition and transcript features between astrocytoma and glioblastoma.
    • Exploratory analyses suggested isoform-level associations with survival, not apparent at the gene level.

    Conclusions:

    • The study presents subtype-specific m6A marking and isoform architecture patterns in glioma, derived from direct RNA sequencing.
    • Findings suggest that m6A variation represents a post-transcriptional regulatory layer critical for glioma biology.
    • While requiring validation, the work provides a hypothesis-generating resource for investigating the glioma epitranscriptome.