TransNet-SAM2: A Transformer-Foundation Model Framework for Prompt-Free Segmentation of White Blood Cells in...

Background: Accurate segmentation of white blood cells (WBCs) in peripheral blood smear images is a fundamental step in computational hematology, enabling downstream tasks such as classification, morphological assessment, and quantitative analysis. However, reliable segmentation remains challenging due to staining variability, complex cellular morphology, overlapping structures, and limited availability of high-quality annotations. Aim and Methods: The aim of this study is to develop a robust and fully automated segmentation framework for white blood cells (WBCs) in microscopic blood smear images, providing a reliable foundation for subsequent computational analysis. We propose TransNet-SAM2, a hybrid deep learning architecture that integrates hierarchical transformer-based feature extraction with a foundation-model-based decoder for prompt-free segmentation. Specifically, a Swin Transformer backbone is employed to capture multi-scale contextual representations, which are subsequently aligned and fused through a feature adaptation module. The fused features are directly injected into the SAM2 mask decoder, replacing conventional prompt-based conditioning and enabling fully automatic segmentation. In addition, a weakly supervised self-training strategy is incorporated to utilize partially annotated data, improving model generalization while reducing annotation requirements. The proposed framework is evaluated using a clinically curated dataset from Dicle University, the publicly available Raabin-WBC dataset, and an additional external leukemic blast validation dataset (ALL-IDB) to assess robustness under both routine and atypical hematological conditions. Results: TransNet-SAM2 achieved a Dice coefficient of 0.95 ± 0.01 and IoU of 0.90 on internal testing, significantly outperforming U-Net (0.89), Mask R-CNN (0.90), and SAM2 (0.92) (p < 0.05). In cross-dataset evaluation (Dicle training, Raabin-WBC testing), the framework maintained strong performance (Dice: 0.91, IoU: 0.84), demonstrating robustness to domain shifts. Ablation studies confirmed each component's contribution, with the full model improving Dice by 6% over a CNN baseline. Qualitative analysis showed accurate boundary delineation even with cell overlap and background clutter. Conclusions: These findings indicate that the proposed framework provides a promising and scalable framework for WBC segmentation. While the current study focuses on segmentation, future work will investigate integration with classification and radiomics pipelines, as well as validation on more diverse clinical datasets, including bone marrow and leukemia samples.