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Responses to Drought and Flooding02:41

Responses to Drought and Flooding

Water plays a significant role in the life cycle of plants. However, insufficient or excess of water can be detrimental and pose a serious threat to plants.

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Divergence of Root Microbiota in Different Habitats based on Weighted Correlation Networks
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Published on: September 25, 2021

Transcriptomic Analyses and Weighted Gene Co-Expression Network Analysis (WGCNA) Identify Key Drought-Responsive

Shengjie Yan1, Zining Jiang2, Xue Liu2

  • 1College of Agronomy, Hunan Agricultural University, Changsha 410128, China.

Plants (Basel, Switzerland)
|June 12, 2026
PubMed
Summary

Drought-tolerant rice (IR65) shows superior root and physiological traits compared to sensitive rice (WAB). Key genes identified in IR65 roots enhance drought tolerance, offering targets for crop improvement.

Keywords:
RNA-seqWGCNAcandidate genesdroughtricerootyeast

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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
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Published on: May 21, 2020

Area of Science:

  • Plant Biology
  • Genetics
  • Biochemistry

Background:

  • Drought is a major threat to global rice production, impacting yield significantly.
  • The root system plays a crucial role in drought perception and tolerance in rice.

Purpose of the Study:

  • To investigate the molecular mechanisms underlying drought tolerance in rice.
  • To compare drought responses between sensitive (WAB) and tolerant (IR65) rice genotypes.

Main Methods:

  • Comparative analysis of shoot and root architectural traits under drought stress (15% PEG6000).
  • Measurement of physio-biochemical parameters including antioxidant enzymes (SOD, CAT, POD, APX), hydrogen peroxide (H2O2), and proline.
  • Transcriptomic analysis (RNA-Seq) to identify differentially expressed genes (DEGs) in roots.
  • Weighted Gene Co-expression Network Analysis (WGCNA) to identify key gene modules.
  • Functional validation of candidate genes in yeast.

Main Results:

  • IR65 exhibited better root and shoot architectural traits and enhanced ROS scavenging and osmotic adjustment capacity than WAB under drought stress.
  • 802 common DEGs were identified in WAB and IR65 roots, enriched in phytohormone signaling, protein phosphorylation, and transcription factors.
  • WGCNA identified nine significant modules, with 12 DEGs upregulated in IR65. Five of these genes conferred tolerance to simulated drought in yeast.

Conclusions:

  • IR65 possesses superior drought tolerance mechanisms at the seedling stage compared to WAB.
  • Specific genes identified in IR65 roots are potential candidates for enhancing rice drought tolerance.
  • These findings provide valuable targets for genetic improvement of rice for drought resilience.