Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Jun 13, 2026

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells
13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

ω-3PUFAs Inhibit Gallbladder Cancer Through Enhancing STK31 Methylation.

Shuang-Xing Li1,2,3, Wei-Yu Zhu1,2,3, Hai-Ying Peng1,2,3

  • 1Central Laboratory of Yan'an Hospital Affiliated to Kunming Medical University, Kunming, Yunnan Province, China.

Molecular Nutrition & Food Research
|June 12, 2026
PubMed
Summary

Related Concept Videos

Cholecystitis01:20

Cholecystitis

Cholecystitis is inflammation of the gallbladder, most commonly caused by obstruction of the cystic duct. This blockage prevents bile from draining, leading to gallbladder distension, inflammation, and potentially serious complications. This condition may present acutely or chronically and can happen with or without gallstones.EtiologyAbout 95% of cholecystitis cases are calculous, caused by gallstones blocking the cystic duct, leading to bile accumulation and inflammation of the gallbladder...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Identification of small-molecule HSF1 amplifiers by high content screening in protection of cells from stress induced injury.

Biochemical and biophysical research communications·2009
Same author

Nanowire transformation by size-dependent cation exchange reactions.

Nano letters·2009
Same author

Effect of haishengsu as an adjunct therapy for patients with advanced renal cell cancer: a randomized and placebo-controlled clinical trial.

Journal of alternative and complementary medicine (New York, N.Y.)·2009
Same author

Identification of inhibitors of HSF1 functional activity by high-content target-based screening.

Journal of biomolecular screening·2009
Same author

Antitumor effects of targeting hTERT lentivirus-mediated RNA interference against KB cell lines.

Oncology research·2009
Same author

Characteristics of emissive spectrum and the removal of nitric oxide in N2/02/NO plasma with argon additive.

Journal of environmental sciences (China)·2009

Omega-3 polyunsaturated fatty acids (ω-3PUFAs) show promise in treating gallbladder cancer (GBC). These fatty acids suppress GBC by epigenetically silencing the STK31 oncogene, offering new therapeutic strategies.

Area of Science:

  • Oncology
  • Molecular Biology
  • Epigenetics

Background:

  • Gallbladder cancer (GBC) is an aggressive malignancy with limited therapeutic options.
  • The potential of omega-3 polyunsaturated fatty acids (ω-3PUFAs) in GBC treatment is largely unexplored.
  • Previous research identified STK31 as a GBC oncogene.

Purpose of the Study:

  • To investigate the anti-GBC effects of ω-3PUFAs (DHA/EPA).
  • To elucidate the molecular mechanisms underlying ω-3PUFAs' action in GBC.
  • To evaluate the therapeutic potential of ω-3PUFAs in preclinical GBC models.

Main Methods:

  • In vitro cell assays and mouse xenograft models were employed.
  • Epigenetic modifications were assessed using Reduced Representation Bisulfite Sequencing (RRBS), Methyl Specific PCR (MSP), and MethyLight PCR.
Keywords:
DNA methylationGSK3βSTK31gallbladder cancerω‐3PUFAs

Related Experiment Videos

Last Updated: Jun 13, 2026

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells
13:38

Synthesis and Characterization of an Aspirin-fumarate Prodrug that Inhibits NFκB Activity and Breast Cancer Stem Cells

Published on: January 18, 2017

  • Downstream signaling pathways were analyzed through proteomics, Co-Immunoprecipitation (Co-IP), and Western blotting.

    • Main Results:

    • ω-3PUFAs significantly suppressed GBC cell proliferation and metastasis in vitro and in vivo.
    • Treatment with ω-3PUFAs induced hypermethylation of the STK31 oncogene promoter, reducing its expression.
    • MethyLight PCR confirmed a significant increase in STK31 promoter methylation from 34.1% to 89.1% in GBC cells.
    • The STK31(DNMT1)/GSK3β pathway and COL4A1 (ITGB1) were identified as key mediators of ω-3PUFAs' anti-GBC effects.
    • Tumor growth inhibition rates of 38.6% and 50.2% were observed in GBC xenografts.

    Conclusions:

    • ω-3PUFAs exert significant anti-GBC effects by epigenetically targeting the STK31 oncogene.
    • This study provides the first evidence of ω-3PUFAs-mediated epigenetic reprogramming in GBC.
    • These findings suggest ω-3PUFAs as a potential dietary intervention or targeted therapy for gallbladder cancer.