Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Multi-layer Cortical Ca²⁺ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Published on: June 13, 2017

CortexCAM: A Camera Array Microscope for Cortex-Wide Cellular Imaging in Freely Locomoting Mice.

Suhasa Kodandaramaiah, Arun Cherkkil, Zoey Viavattine

Research Square

|June 12, 2026

Summary

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Coordinated Representational Drift Across the Mouse Cortex.

Research square·2026

Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

Nature methods·2026

Same author

Chromatix: a differentiable, GPU-accelerated wave-optics library.

bioRxiv : the preprint server for biology·2026

Same author

Cell-MICS: Detecting Immune Cells With Label-Free Two-Photon Autofluorescence and Deep Learning.

Journal of biophotonics·2026

Same author

Cortex-wide characterization of decision-making neural dynamics during spatial navigation.

Nature communications·2026

Same author

Cortex-Wide Cellular Imaging in Freely Locomoting Mice Using Cortex Camera Array Microscope (CortexCAM).

bioRxiv : the preprint server for biology·2026

Same journal

HIV Transmission Dynamics in Greater Mexico City are Shaped by Dense Spatial Mixing.

Research square·2026

Same journal

A UCP1-IRES-Cre Knock-In Mouse Enables Specific Brown Adipocyte Targeting Without CNS Off-Target Expression.

Research square·2026

Same journal

Precision RNAi for Fibrodysplasia Ossificans Progressiva: a combinatorial, unimolecular, allele selective approach.

Research square·2026

Same journal

Perceptions of end-of-life care quality among bereaved closest contacts of community-dwelling older Australians: a cross-sectional survey of the ASPREE cohort.

Research square·2026

Same journal

Heavy-chain immune repertoire sequencing enables language-model prediction of antigen-specific antibodies.

Research square·2026

Same journal

25+ Years of TRPV4: From Discovery to Translational Horizons.

Research square·2026

See all related articles

This summary is machine-generated.

Researchers developed the Cortex Camera Array Microscope (CortexCAM) for simultaneous, wide-scale neuronal activity imaging. This system enables studying brain-wide cellular dynamics in freely moving mice during natural behaviors.

Area of Science:

Systems Neuroscience
Neuroimaging
Cellular Neuroscience

Background:

Understanding neuronal activity across brain regions during natural behaviors is crucial.
Current methods often limit simultaneous imaging to small brain areas or require head-fixed preparations.

Purpose of the Study:

To introduce a novel imaging system, the Cortex Camera Array Microscope (CortexCAM), for large-scale, simultaneous neuronal activity recording.
To enable the study of cortex-wide neural dynamics in freely behaving animals during ethologically relevant tasks.

Main Methods:

Developed the Cortex Camera Array Microscope (CortexCAM) integrating four miniaturized fluorescence microscopes.
Integrated CortexCAM into a mobile gantry system for volitional motion control (translational and rotational).

More Related Videos

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents

Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents

Published on: June 29, 2014

Related Experiment Videos

Last Updated: Jun 13, 2026

Multi-layer Cortical Ca2+ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Multi-layer Cortical Ca²⁺ Imaging in Freely Moving Mice with Prism Probes and Miniaturized Fluorescence Microscopy

Published on: June 13, 2017

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Large-scale Three-dimensional Imaging of Cellular Organization in the Mouse Neocortex

Published on: September 5, 2018

Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents

Flat-floored Air-lifted Platform: A New Method for Combining Behavior with Microscopy or Electrophysiology on Awake Freely Moving Rodents

Published on: June 29, 2014

Enabled imaging of over 9000 neurons across dorsal cortical regions in freely locomoting mice.

Main Results:

Achieved simultaneous, cortex-wide cellular resolution imaging over 48 mm2 of the dorsal cortex.
Successfully imaged neuronal activity in motor, somatosensory, visual, retrosplenial, and association cortices.
Demonstrated imaging during volitional movement, choice tasks, and social interactions in mice.

Conclusions:

The mobile CortexCAM system facilitates studying brain-wide neural dynamics during naturalistic behaviors.
This technology overcomes limitations of head-fixed preparations for systems neuroscience research.
Enables novel insights into neural circuits underlying complex behaviors.