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Updated: Jun 13, 2026

Measuring the Rate of Lipolysis in Ex Vivo Murine Adipose Tissue and Primary Preadipocytes Differentiated In Vitro
Published on: March 17, 2023
PPAR-δ is a potential modulator of central carbon metabolism in human adipocytes
Leilei Sun1, Martin Wabitsch2, Jian Yang1
1Department of Pharmaceutical and Nutrition Sciences, Research Group, College of Pharmacy and Nutrition, University of Saskatchewan, 107 Wiggins Road, Saskatoon, SK S7N 5E5, Canada.
Abstract:
Adipogenesis involves adipocyte differentiation and synthesis and storage of fats. PPAR-γ is the master regulator of adipogenesis and regulates genes for adipocyte differentiation, lipogenesis, adipocyte survival and adipokine secretion. Central carbon metabolism (CCM) comprises of three key pathways glycolysis, tricarboxylic acid (TCA) cycle and pentose phosphate pathway (PPP). CCM utilizes carbon sources to provide energy and building blocks for lipogenesis. Several targets of transcription factor PPAR-γ have been identified in CCM pathways. However, the exact mechanism of PPAR-γ modulation in adipogenesis via CCM remains elusive. Therefore, it is of interest to evaluate the effects of PPAR-γ on CCM in differentiated human SGBS adipocytes by real time-qPCR and metabolomics using its agonist 15d-PGJ2 and antagonist GW9662. Surprisingly, our results suggest that PPAR-δ rather than PPAR-γ is likely to be the key modulator of CCM in differentiated adipocytes at least under current experimental conditions with both 15d-PGJ2 and GW9662 being PPAR-δ agonists, although PPAR-γ plays a crucial role in adipogenesis and lipogenesis. This data provides insight to manage obesity and diabetes using PPAR-δ agonists. Further studies are warranted to explore the regulation of CCM by PPARs in adipocytes.
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