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Updated: Jun 13, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Published on: December 9, 2022

Organic acid-based deep eutectic solvents (DESs) are potent antimicrobial agents against antibiotic-resistant

Mohamed Abdelfattah Maky^1,2, Yanath Belguesmia¹, Chérifa Obone Opimba³

¹UMR Transfrontalière BioEcoAgro INRAe 1158, Université de Lille, 59000 Lille, France.

Current Research in Microbial Sciences

|June 12, 2026

Summary

Organic acid-based deep eutectic solvents (DESs) show potent antimicrobial activity against antibiotic-resistant bacteria and viruses. These novel DESs effectively eradicated biofilms and reduced viral titres, offering promising antimicrobial candidates.

Keywords:

Antibacterial Antivirals Biofilms Cytotoxicity Deep eutectic solvents Mechanism Polymer nanoparticles

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Preparation of Binary and Ternary Deep Eutectic Systems

Preparation of Binary and Ternary Deep Eutectic Systems

Published on: October 31, 2019

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Last Updated: Jun 13, 2026

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Evaluation of the Efficacy of Organic Peroxyacids for Eradicating Dairy Biofilms Using an Approach Combining Static and Dynamic Methods

Published on: December 9, 2022

Preparation of Binary and Ternary Deep Eutectic Systems

Preparation of Binary and Ternary Deep Eutectic Systems

Published on: October 31, 2019

Area of Science:

Antimicrobial drug discovery
Green chemistry
Nanotechnology

Background:

Global health is threatened by rising antibiotic resistance, biofilms, and viral contamination in healthcare and food industries.
Deep eutectic solvents (DESs) are emerging as potential antimicrobial agents, but their efficacy against resistant strains and viruses is largely uncharacterized.

Purpose of the Study:

To evaluate the antimicrobial and antiviral effectiveness of organic acid-based and non-organic acid-based DESs.
To investigate the synergistic effects of DESs with nanoparticles against resistant bacteria and biofilms.

Main Methods:

Tested four DESs (T-glycoline, oxaline, reline, glyceline) against antibiotic-resistant Gram-positive/negative bacteria and enveloped/non-enveloped viruses.
Determined minimum inhibitory concentrations (MICs) and assessed synergy with nanoparticles (e.g., alginate/starch) against MRSA and Enterobacterales.
Utilized confocal microscopy and transmission electron microscopy (TEM) to visualize bacterial cell death and cytoplasmic disruption.

Main Results:

Organic acid-based DESs (T-glycoline, oxaline) exhibited superior activity (MICs 0.20-0.83% v/v) compared to non-organic DESs.
T-glycoline and oxaline showed synergy against colistin-resistant Enterobacterales, downregulating the mcr gene.
Combined T-glycoline with alginate/starch nanoparticles enhanced efficacy against MRSA and improved Caco-2 cell viability.
Organic DESs effectively eradicated mature biofilms and significantly reduced infectious viral titres.

Conclusions:

Selected organic acid-based DES formulations demonstrate significant potential as novel antimicrobial and antiviral agents.
Synergistic combinations with nanoparticles enhance DES efficacy and improve cell viability, suggesting tailored applications.
Further research into the safety profile of these DESs is crucial for their practical implementation in healthcare and food industries.