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Related Concept Videos

In vitro Mutagenesis01:16

In vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.
In-vitro Mutagenesis01:16

In-vitro Mutagenesis

To learn more about the function of a gene, researchers can observe what happens when the gene is inactivated or “knocked out,” by creating genetically engineered knockout animals. Knockout mice have been particularly useful as models for human diseases such as cancer, Parkinson’s disease, and diabetes.

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Related Experiment Video

Updated: Jun 13, 2026

An Array-based Comparative Genomic Hybridization Platform for Efficient Detection of Copy Number Variations in Fast Neutron-induced Medicago truncatula Mutants
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KitBase Expanded: An Integrated Genomic and Phenotypic Resource for 3,268 Fast-Neutron-Irradiated Rice Mutants.

Artur Teixeira de Araujo1,2,3, Rashmi Jain1,2, Deling Ruan1,2

  • 1Department of Plant Pathology, University of California, Davis, CA, United States.

Database : the Journal of Biological Databases and Curation
|June 12, 2026
PubMed
Summary
This summary is machine-generated.

Fast-neutron mutagenesis in rice generated over 428,000 mutations across thousands of lines, enabling the discovery of genes influencing important agronomic traits. This resource facilitates crop improvement through functional genomics.

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Area of Science:

  • Plant Biology
  • Genomics
  • Crop Science

Background:

  • Fast-neutron (FN) mutagenesis is a key method for generating genome-wide mutations in crop plants.
  • Understanding the genetic basis of agronomic traits is crucial for crop improvement.

Purpose of the Study:

  • To conduct a comprehensive genomic and phenotypic analysis of 3268 FN-induced mutant rice lines (Oryza sativa L. cv. Kitaake).
  • To catalog mutations, analyze their distribution, and identify candidate genes for various agronomic traits.
  • To update and provide open access to the KitBase platform for rice functional genomics.

Main Methods:

  • Whole-genome sequencing of 3268 FN mutant rice lines.
  • Mutation identification through alignment to Nipponbare and KitaakeX reference genomes.
  • Phenotypic characterization of over 2700 lines for core agronomic traits.
  • Integration of genomic and phenotypic data via the KitBase platform.

Main Results:

  • Cataloged over 428,000 mutations affecting a significant percentage of genes in both reference genomes.
  • Identified an average of 63-68 mutations per line, randomly distributed across chromosomes.
  • Observed broad phenotypic variation in agronomic traits and identified candidate genes for several traits.
  • Found that 74.4% of Nipponbare transcription factor genes were mutated.

Conclusions:

  • The expanded FN mutant resource provides a valuable tool for rice functional genomics.
  • Coupling high-density mutation mapping with phenotypic data accelerates gene discovery and crop improvement.
  • The KitBase platform offers open access to data and resources, facilitating genetic analyses.