Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Liposome array on a power-free microfluidic device for analysis of nanopore formation.

The Analyst·2025

Same author

Paper-Based Analytical Device Coated with a Fluorescent Polymer for the Visual Detection of Biothiols.

Langmuir : the ACS journal of surfaces and colloids·2025

Same author

Refractory Condyloma Acuminatum in Two Pediatric Patients.

Cureus·2025

Same author

Isolation of SSEA-3-positive mesenchymal stem cells from equine bone marrow and evaluation of their pluripotency.

Research in veterinary science·2025

Same author

Microfluidic devices integrated with track-etched porous membranes for cell-based bioanalysis.

Analytical sciences : the international journal of the Japan Society for Analytical Chemistry·2025

Same author

Evaluation of Remote Surgical Hands-on Training in Veterinary Education Using a Hololens Mixed Reality Head-Mounted Display.

Journal of veterinary medical education·2024

Same journal

Expanding CAM Models: In Vivo PET/CT Imaging of Cancer Xenografts on the Ostrich CAM.

ACS applied bio materials·2026

Same journal

Photothermal Pulse Output-Nanomachines for Enhanced Cuproptosis in Tumor Cells.

ACS applied bio materials·2026

Same journal

TENG-Driven Electrotherapy: A Self-Powered Approach to Inducing Cancer Cell Apoptosis.

ACS applied bio materials·2026

Same journal

Heparinized Elastomeric Nanofibrillar Grafts: Mechanically Tunable, Cell-Supportive, and Thromboresistant Vascular Substitutes.

ACS applied bio materials·2026

Same journal

Adenosine Triphosphate-Coated Gold Nanoclusters as Evolutionary Adjuvants Suppress <i>fusA</i>-Mediated Antibiotic Resistance Evolution.

ACS applied bio materials·2026

Same journal

Localized Nitric Oxide Release from a Ciprofloxacin Hybrid Conjugate Enables Potent Disruption of Gram-Positive and Gram-Negative Biofilms.

ACS applied bio materials·2026

See all related articles

Search research articles

Related Experiment Video

Updated: Jun 13, 2026

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

Published on: March 17, 2023

Coating-Free Methods for Forming Liposomes Using Hydrophilic/Hydrophobic Composite Microfluidic Device.

Yiting Zhang¹, Naoki Sasaki¹

¹Department of Chemistry, College of Science, Rikkyo University, 3-34-1 Nishi-Ikebukuro, Toshima, Tokyo 171-8501, Japan.

ACS Applied Bio Materials

|June 12, 2026

Summary

This summary is machine-generated.

Researchers developed a simple microchannel device for creating uniform liposomes. This fabrication method offers a streamlined approach for liposome production and characterization in microfluidic systems.

Keywords:

coating-free giant unilamellar vesicle hydrophilic agent liposome microfluidics polydimethylsiloxane wettability

More Related Videos

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface

Published on: May 1, 2020

Related Experiment Videos

Last Updated: Jun 13, 2026

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

On-Chip Octanol-Assisted Liposome Assembly for Bioengineering

Published on: March 17, 2023

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Automated Lipid Bilayer Membrane Formation Using a Polydimethylsiloxane Thin Film

Published on: July 10, 2016

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface

Self-Assembly of Hybrid Lipid Membranes Doped with Hydrophobic Organic Molecules at the Water/Air Interface

Published on: May 1, 2020

Area of Science:

Biomaterials Science
Microfluidics
Nanotechnology

Background:

Liposomes are crucial drug delivery vehicles.
Microfluidic devices offer precise control over liposome formation.
Current fabrication methods can be complex and difficult to scale.

Purpose of the Study:

To develop a simplified and efficient method for fabricating a device for liposome formation in microchannels.
To optimize the fabrication process for reliable liposome production.
To characterize the size uniformity of the generated liposomes.

Main Methods:

Fabrication of a microfluidic device using patterned hydrophilic and hydrophobic polydimethylsiloxane on a mold substrate.
Optimization of the device fabrication process.
Evaluation of liposome formation using fluorescence microscopy.
Confirmation of liposome integrity by dye encapsulation and lipid bilayer visualization.

Main Results:

Successful fabrication of a device enabling liposome formation in microchannels.
Confirmation of liposome formation through fluorescence microscopy, showing encapsulated dye and lipid bilayers.
Achieved good size uniformity in the collected liposomes, with a coefficient of variation of 13%.

Conclusions:

The simplified device fabrication strategy is effective for producing liposomes in microchannels.
The developed method yields liposomes with high size uniformity.
This approach presents a promising platform for scalable and controlled liposome generation.