Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nonsense-mediated mRNA Decay02:27

Nonsense-mediated mRNA Decay

The Upf proteins that carry out nonsense-mediated decay (NMD) are found in all eukaryotic organisms, including humans. Each protein has an individual role, but they need to work in collaboration. Upf1 is an ATP-dependent RNA helicase that unwinds the RNA helix. Because Upf1 can unwind any RNA, Upf2 and Upf3 are required to help Upf1 discriminate between nonsense and normal mRNAs.
Usually, Upf3 binds to an Exon Junction Complex (EJC) at mRNA splice sites. If a ribosome fully translates the mRNA,...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
Nuclear Export of mRNA02:31

Nuclear Export of mRNA

Before mRNAs are exported to the cytoplasm, it is crucial to check each mRNA for structural and functional integrity. Eukaryotic cells use several different mechanisms, collectively known as mRNA surveillance, to look for irregularities in mRNAs. Irregular or aberrant mRNA are rapidly degraded by various enzymes. If a defective mRNA escapes the surveillance, it would be translated into a protein which would either be non-functional or not function properly. One of the primary irregularities in...
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability
mRNA Stability and Gene Expression02:51

mRNA Stability and Gene Expression

The structure and stability of mRNA molecules regulates gene expression, as mRNAs are a key step in the pathway from gene to protein. In eukaryotes, the half-life of mRNA varies from a few minutes up to several days. mRNA stability is essential in growth and development. The absence of the proteins regulating its stability, such as tristetraprolin in mice, can cause systemic issues, including bone marrow overgrowth, inflammation, and autoimmunity.
Cis-acting Elements involved in mRNA stability

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

How oxidized EGCG remodels α-synuclein fibrils into non-toxic aggregates: insights from computational simulations.

Physical chemistry chemical physics : PCCP·2023
Same author

Green Tea Polyphenol Microparticles Based on the Oxidative Coupling of EGCG Inhibit Amyloid Aggregation/Cytotoxicity and Serve as a Platform for Drug Delivery.

ACS biomaterials science & engineering·2021
Same author

Acetylsalicylic acid and salicylic acid present anticancer properties against melanoma by promoting nitric oxide-dependent endoplasmic reticulum stress and apoptosis.

Scientific reports·2020
Same author

Selective and Sensitive Pull Down of Amyloid Fibrils Produced in Vitro and in Vivo by the Use of Pentameric-Thiophene-Coupled Resins.

ACS chemical neuroscience·2018
Same author

Protein charge distribution in proteomes and its impact on translation.

PLoS computational biology·2017
Same author

An ortho-Iminoquinone Compound Reacts with Lysine Inhibiting Aggregation while Remodeling Mature Amyloid Fibrils.

ACS chemical neuroscience·2017
Same journal

Evaluating the effect of γ-oryzanol on MASLD pathology using a medaka fish model.

FEBS open bio·2026
Same journal

Dose-dependent hepatotoxicity of hydrogen peroxide in HepG2 cells and its modulation by CYP450 induction.

FEBS open bio·2026
Same journal

How phagocytic cells kill bacteria: Lessons from a professional killer.

FEBS open bio·2026
Same journal

MagmaFlow: A desktop platform for artificial intelligence-driven expression analysis.

FEBS open bio·2026
Same journal

A new flow chip in combination with multiphoton microscopy as a protocol for longitudinal 3D imaging of tissue calcification under shear stress.

FEBS open bio·2026
Same journal

Effect of terahertz irradiation on DNA damage repair in living cells.

FEBS open bio·2026
See all related articles

Related Experiment Video

Updated: Jun 13, 2026

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
07:03

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

Transcripts enriched in codons that trigger P-site tRNA-mediated mRNA decay possess stable mRNA.

Rodolfo Lopes Carneiro1, Fernando Lucas Palhano1

  • 1Programa de Biologia Estrutural, Instituto de Bioquímica Médica Leopoldo de Meis, Universidade Federal do Rio de Janeiro, Brazil.

FEBS Open Bio
|June 12, 2026
PubMed
Summary
This summary is machine-generated.

Human P-site tRNA-mediated decay (PTMD) selectively reduces the stability of long-lived transcripts, unlike yeast mRNA decay mechanisms. This distinct process involves specific codons and CNOT3, highlighting a novel regulatory role in gene expression.

Keywords:
Translationcodon usagemRNA decaymRNA turnovertRNA

More Related Videos

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
12:21

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Published on: December 13, 2014

Related Experiment Videos

Last Updated: Jun 13, 2026

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts
07:03

Determining Genome-wide Transcript Decay Rates in Proliferating and Quiescent Human Fibroblasts

Published on: January 2, 2018

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells
06:48

A Reporter Assay to Analyze Intronic microRNA Maturation in Mammalian Cells

Published on: June 16, 2022

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains
12:21

Measurement of mRNA Decay Rates in Saccharomyces cerevisiae Using rpb1-1 Strains

Published on: December 13, 2014

Area of Science:

  • Molecular Biology
  • Genetics
  • Gene Regulation

Background:

  • Synonymous codon usage impacts mRNA stability and decay in yeast via the CCR4-NOT complex.
  • Codon-mediated mRNA decay in humans is less understood, with recent identification of P-site tRNA-mediated decay (PTMD).

Purpose of the Study:

  • To investigate the characteristics of human transcripts affected by PTMD.
  • To elucidate the distinct regulatory role of PTMD in human gene expression compared to yeast.

Main Methods:

  • Analysis of public transcriptomic datasets.
  • Identification of transcripts enriched in PTMD-associated codons (specific arginine codons at P-site).

Main Results:

  • Human PTMD involves specific arginine codons (CGG, CGA, AGG) at the P site and slow A-site decoding.
  • Transcripts enriched in PTMD codons were found to have longer half-lives.
  • PTMD selectively targets and reduces the stability of otherwise long-lived human transcripts.

Conclusions:

  • Human PTMD represents a distinct mRNA decay mechanism from yeast, focusing on stabilizing long-lived transcripts.
  • PTMD, involving CNOT3, plays a regulatory role in human gene expression by modulating the stability of specific mRNA populations.