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Updated: Jun 13, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Synthetic Substrate Discovery for Hck Kinase via Phage Display.

Akaash Kannan¹, Jason L Heier¹, Dhruvi P Motwani¹

¹University of Minnesota, Department of Biochemistry, Molecular Biology and Biophysics, 420 Washington Avenue SE, Minneapolis, Minnesota 55455, United States.

|June 12, 2026

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We developed a new phage display method to identify protein tyrosine kinase substrates. This high-throughput approach precisely profiles kinase activity and reveals novel substrates with strong kinetic properties.

Area of Science:

Biochemistry
Molecular Biology
Oncology

Background:

Protein tyrosine kinases (PTKs) are crucial for cell signaling, and their dysregulation is linked to cancer.
Understanding PTK substrate specificity is vital for cancer research but current methods lack scale and resolution.

Purpose of the Study:

To develop a high-throughput method for comprehensive profiling of PTK substrates.
To identify novel substrates and understand kinase-substrate recognition for the Hck kinase.

Main Methods:

Utilized a modernized phage display platform integrated with next-generation sequencing.
Performed simultaneous assessment of billions of potential substrates for Hck kinase.
Analyzed positional and motif enrichment for iterative substrate design.

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Related Experiment Videos

Last Updated: Jun 13, 2026

Identification of Kinase-substrate Pairs Using High Throughput Screening

Identification of Kinase-substrate Pairs Using High Throughput Screening

Published on: August 29, 2015

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Using Phage Display to Develop Ubiquitin Variant Modulators for E3 Ligases

Published on: August 27, 2021

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Identification of Novel CK2 Kinase Substrates Using a Versatile Biochemical Approach

Published on: February 21, 2019

Main Results:

Successfully profiled Hck kinase substrates at an unprecedented scale and resolution.
Identified novel substrates with robust kinetic behavior (K_m < 30 μM).
Gained insights into Hck substrate preferences in multipositional sequence contexts.

Conclusions:

The new phage display platform offers a powerful tool for PTK substrate profiling.
This method advances our understanding of kinase-substrate interactions and cancer biology.
The identified substrates and insights can inform future therapeutic strategies targeting PTKs.