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Adjunctive thymosin beta-4 (Tβ4) with ciprofloxacin significantly improves vision and corneal nerve regeneration in bacterial keratitis. This combination therapy restores nerve integrity and visual function, offering a promising treatment strategy.

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Area of Science:

  • Ophthalmology
  • Regenerative Medicine
  • Microbiology

Background:

  • Bacterial keratitis can lead to long-term vision impairment.
  • Thymosin beta-4 (Tβ4) combined with ciprofloxacin has shown promise in reducing keratitis severity and enhancing wound repair.
  • The impact of Tβ4 on corneal nerves and visual function post-infection is not well understood.

Purpose of the Study:

  • To evaluate the efficacy of adjunctive thymosin beta-4 (Tβ4) in supporting corneal nerve regeneration.
  • To determine if Tβ4 treatment can restore visual function after bacterial keratitis.
  • To assess the combined effect of Tβ4 and ciprofloxacin on corneal nerve integrity and vision.

Main Methods:

  • Bacterial keratitis was induced in mice using Pseudomonas aeruginosa.
  • Mice received topical treatments including PBS, Tβ4 monotherapy, ciprofloxacin monotherapy, or adjunctive Tβ4 + ciprofloxacin.
  • Visual acuity, contrast sensitivity, corneal sensitivity, and corneal nerve density (β-III tubulin immunofluorescence) were assessed.

Main Results:

  • Adjunctive Tβ4 + ciprofloxacin significantly improved visual acuity and contrast sensitivity compared to monotherapies.
  • Combination therapy markedly enhanced corneal sensitivity and nerve regeneration.
  • Nerve density and architecture were restored to levels comparable to uninfected controls.

Conclusions:

  • Adjunctive Tβ4 treatment promotes significant visual function and corneal nerve regeneration in bacterial keratitis.
  • Combination therapy with Tβ4 and ciprofloxacin restores both functional vision and corneal nerve integrity.
  • This study supports the development of Tβ4-based therapies and highlights the importance of assessing nerve regeneration and visual function in treatment evaluations.