Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Inorganic Nitrogen Assimilation01:22

Inorganic Nitrogen Assimilation

Nitrogen is an essential element in biological systems, forming a crucial component of proteins, nucleic acids, and other cellular constituents. Many bacteria and archaea acquire nitrogen in the form of nitrate (NO₃⁻) or ammonia (NH₃), which are then assimilated into biomolecules through specific enzymatic pathways.Assimilatory Nitrate ReductionWhen nitrate enters the cell, it undergoes a two-step reduction process known as assimilatory nitrate reduction. Initially, the enzyme nitrate reductase...
Microbial Wastewater Treatment01:30

Microbial Wastewater Treatment

Microbial communities in aquatic ecosystems play a key role in the natural breakdown of contaminants introduced through domestic and industrial effluents. Acting as biological catalysts, these microbes change and mineralize a wide range of organic and inorganic pollutants under different redox conditions.In oxygen-rich surface waters, aerobic heterotrophs lead organic matter breakdown, using oxygen as the terminal electron acceptor to efficiently oxidize substrates to carbon dioxide and water.
Microbes and the Nitrogen Cycle01:26

Microbes and the Nitrogen Cycle

The nitrogen cycle is a complex biogeochemical process critical to maintaining the balance of nitrogenous compounds in ecosystems. This cycle involves multiple microbial-mediated transformations through which nitrogen changes oxidation states, supporting essential ecological functions and contributing to plant and microbial growth.Nitrogen Fixation and AmmonificationNitrogen fixation initiates the cycle by converting inert atmospheric nitrogen (N₂) into bioavailable ammonia (NH₃), a process...
Metabolism of Chemolithotrophs01:15

Metabolism of Chemolithotrophs

Chemolithotrophs are microorganisms that obtain energy by oxidizing inorganic molecules such as hydrogen gas (H₂), ammonia (NH₃), reduced sulfur compounds (H₂S, S²⁻), and ferrous iron (Fe²⁺). Unlike heterotrophic organisms that rely on organic carbon, chemolithotrophs transfer electrons from these inorganic donors to the electron transport chain (ETC), generating a proton motive force (PMF) that drives ATP synthesis through oxidative phosphorylation. However, because inorganic electron donors...
Microbial Mats01:25

Microbial Mats

Microbial communities forming biofilms and mats represent complex, spatially structured ecosystems where metabolic processes are stratified according to light, oxygen, and nutrient gradients. Biofilms are initial colonization stages, only a few millimeters thick, while mature microbial mats can reach centimeter-scale thickness and display intricate vertical organization. Their structural and functional heterogeneity allows microorganisms to occupy distinct ecological niches within a few...
The Nitrogen Cycle01:49

The Nitrogen Cycle

Nitrogen atoms, present in all proteins and DNA, are recycled between abiotic and biotic components of the ecosystem. However, the primary form of nitrogen on Earth is nitrogen gas, which cannot be used by most animals and plants. Thus, nitrogen gas must first be converted into a usable form by nitrogen-fixing bacteria before it can be cycled through other living organisms. The use of nitrogen-containing fertilizers and animal waste products in human agriculture has greatly influenced the...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Exploring seasonal lability and fate of dissolved organic nitrogen cycling in a field-scale water filtration system.

Water research·2026
Same author

Supra-Permafrost Groundwater is a Source of Highly Biodegradable Dissolved Organic Matter to the Arctic Ocean.

Environmental science & technology·2026
Same author

The molecular-level diagenetic clock of sinking marine organic matter.

Proceedings of the National Academy of Sciences of the United States of America·2025
Same author

Characterization of Burned Soil Organic Matter via Sequential Solvent Extractions and 21 T FT-ICR Mass Spectrometry with Electrospray and Atmospheric Pressure Photoionization.

Analytical chemistry·2025
Same author

The 34th Sanibel Conference on Mass Spectrometry: Mass Spectrometry in Energy and the Environment.

Journal of the American Society for Mass Spectrometry·2025
Same author

Detection and Structural Elucidation of Copper Binding Tri- and Tetrapyrrole Ligands Produced by the Marine Diatom <i>Phaeodactylum Tricornutum</i>.

Journal of the American Society for Mass Spectrometry·2025

Related Experiment Video

Updated: Jun 13, 2026

Understanding Dissolved Organic Matter Biogeochemistry Through In Situ Nutrient Manipulations in Stream Ecosystems
09:38

Understanding Dissolved Organic Matter Biogeochemistry Through In Situ Nutrient Manipulations in Stream Ecosystems

Published on: October 29, 2016

Exploring Molecular Dynamics and Structure Change of Dissolved Organic Nitrogen under Nitrate and Phosphate Pulses in

Jinxiang Cheng1, Alejandra Robles-Lecompte1, Amy M McKenna2

  • 1Department of Civil, Environmental, and Construction Engineering, University of Central Florida, 4000 Central Florida Blvd, Orlando, Florida 32816, United States.

Environmental Science & Technology
|June 12, 2026
PubMed
Summary
This summary is machine-generated.

Controlling nitrogen and phosphorus ratios in stormwater influent and selecting specific filter media significantly alters the chemical makeup and removal of dissolved organic nitrogen (DON). This impacts nutrient cycling and treatment effectiveness.

Keywords:
cascade upflow biofiltration systemdissolved organic nitrogenmolecular dynamicsnitrate and phosphate pulsesstructure change

More Related Videos

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
08:13

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Related Experiment Videos

Last Updated: Jun 13, 2026

Understanding Dissolved Organic Matter Biogeochemistry Through In Situ Nutrient Manipulations in Stream Ecosystems
09:38

Understanding Dissolved Organic Matter Biogeochemistry Through In Situ Nutrient Manipulations in Stream Ecosystems

Published on: October 29, 2016

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O
08:05

Measurement of the Potential Rates of Dissimilatory Nitrate Reduction to Ammonium Based on 14NH4+/15NH4+ Analyses via Sequential Conversion to N2O

Published on: October 7, 2020

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities
08:13

A Novel Bioreactor for High Density Cultivation of Diverse Microbial Communities

Published on: December 25, 2015

Area of Science:

  • Environmental chemistry
  • Water treatment technologies
  • Biogeochemical cycling

Background:

  • Dissolved organic nitrogen (DON) is a significant and reactive component of stormwater nitrogen that is often poorly managed.
  • Understanding DON's fate is crucial for effective stormwater treatment and mitigating downstream nitrogen pollution.

Purpose of the Study:

  • To investigate how influent nitrogen:phosphorus (N:P) stoichiometry and porous media composition influence the transformation and removal of dissolved organic nitrogen (DON) in a bioreactor system.
  • To characterize the molecular changes in DON under different treatment conditions and media types.

Main Methods:

  • Utilized a cascade upflow bioreactor packed with two distinct adsorbent media: BIPGEM and ZIPGEM.
  • Applied short pulses of nitrate alone (AC1) or nitrate plus phosphate (AC2) to the reactors.
  • Analyzed influent and effluent samples using high-resolution 21 T Fourier transform ion cyclotron resonance mass spectrometry to determine the molecular formulas of nitrogen-containing compounds (CHON formulas).

Main Results:

  • Influent N:P stoichiometry reprogrammed produced DON chemistry in a media-specific manner.
  • BIPGEM produced heavier, less labile DON under AC2, while ZIPGEM produced more oxidized, labile DON and preferentially removed unsaturated formulas.
  • Both media showed moderate nitrate removal, but ZIPGEM exhibited stronger phosphate removal under AC2, whereas BIPGEM released phosphate under AC1.

Conclusions:

  • Influent N:P stoichiometry and the choice and arrangement of porous media are critical factors for controlling nutrient removal and the quality of residual DON.
  • These findings offer insights into optimizing stormwater treatment strategies for improved nitrogen management and reduced downstream impacts.