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  1. Home
  3. Ready-to-load Mhc-i Nanoparticles For High-throughput T Cell Screening Studies.
  1. Home
  3. Ready-to-load Mhc-i Nanoparticles For High-throughput T Cell Screening Studies.

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Related Experiment Video

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

Ready-to-load MHC-I nanoparticles for high-throughput T cell screening studies.

Hoang Anh T Phan1,2,3, Soomin Eom1,3, Daniel Hwang1,3

  • 1Center for Computational and Genomic Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.

Science Advances
|June 12, 2026

View abstract on PubMed

Summary
This summary is machine-generated.

We developed VLP-Open HLA, a novel platform using virus-like particles to display peptide-MHC molecules. This tool aids in identifying T cell receptors (TCRs) and activating T cells for immunology research.

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Related Experiment Videos

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes
07:59

A High Throughput MHC II Binding Assay for Quantitative Analysis of Peptide Epitopes

Published on: March 25, 2014

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules
08:43

A Fluorescence-based Lymphocyte Assay Suitable for High-throughput Screening of Small Molecules

Published on: March 10, 2017

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles
09:28

Enrich and Expand Rare Antigen-specific T Cells with Magnetic Nanoparticles

Published on: November 17, 2018

Area of Science:

  • Immunology
  • Molecular Biology
  • Biotechnology

Background:

  • Major Histocompatibility Complex (MHC) class I proteins present peptides to CD8+ T cells, crucial for adaptive immunity.
  • T cell receptor (TCR) interactions with peptide-MHC (pMHC) influence T cell activation or anergy.
  • Understanding these interactions is key for developing immunotherapies and vaccines.

Purpose of the Study:

  • To introduce VLP-Open HLA, a novel multivalent platform for displaying peptide-MHC molecules.
  • To establish VLP-Open HLA as a tool for screening antigen-specific CD8+ T cells and orphan TCRs.
  • To demonstrate VLP-Open HLA's utility as an artificial antigen-presenting system for T cell activation and expansion.

Main Methods:

  • Development of a virus-like particle (VLP) platform capable of displaying up to 60 peptide/HLA molecules.
  • Utilizing VLP-Open HLA for staining antigen-specific CD8+ T cells.
  • Employing VLP-Open HLA with costimulation as an artificial antigen-presenting platform.

Main Results:

  • VLP-Open HLA successfully displays a high density of peptide-MHC molecules.
  • The platform enables efficient screening of antigen-specific CD8+ T cells and identification of orphan TCRs.
  • VLP-Open HLA combined with costimulation effectively induces antigen-specific T cell activation and expansion.

Conclusions:

  • VLP-Open HLA offers a versatile and efficient platform for immunological research.
  • It serves as a valuable tool for TCR screening and artificial antigen presentation.
  • The platform's adaptability to various HLA allotypes and costimulatory molecules expands its potential applications in experimental immunology.